Fig 1: Pt(S-pr-thiosal)2 enhances expression of proapoptotic molecules, while reducing expression of antiapoptotic molecules in BCL1 cells. (a) The graphs show the mean for the percentage of Noxa and Bcl2 positive BCL1 cells. The statistical significance of the difference in the percentage of apoptotic cells was determined by Student’s t test; * p < 0.05, ** p < 0.01 and indicates the difference in the percentage of BCL1 cells treated with platinum complex at 0.05 mg/mL and all three other groups of BCL1 cells (untreated; treated with cisplatin; and treated with platinum complex at 0.01 mg/mL). (b) Representative histograms show the expression of Bcl-2 and Noxa in untreated, cisplatin-treated, and Pt(S-pr-thiosal)2 treated BCL1 cells; (c) Mean values +SD for expression of mRNA for Bcl2, Bax and caspase-3 detected in BCL1 cells by real-time qRT-PCR for 5 samples per group are shown. The statistical significance was determined by Student’s t test; * p < 0.05, ** p < 0.01. (d) Immunofluorescence staining for (green stained proteins Bcl-2 (left), Bax (middle), and active form of caspase 3 (right); DNA stained blue) (DAPI)) in BCL1 cells untreated and treated with platinum complex for 12 h at a dose of 0.05 mg/mL, magnification 200×.