Fig 1: Strategies for enrichment of HSPC(A) Human Lin- cells can be enriched using a magnetic stand. Left. Cells are incubated with the progenitor cell enrichment cocktail followed by incubation with magnetic particles and incubation in the magnetic stand. Right. Lin+ cells bound by the antibodies of the enrichment cocktail and by the magnetic particles are attracted to the tube wall by the magnetic field. Lin- cells remain in suspension and can be recovered by simply pouring off the liquid into a new tube. The Lin+ cells remain in the original tube.(B) Mouse and human HSPCs can be enriched using columns for magnetic cell separation. Cells are incubated with cell enrichment cocktail followed by incubation with magnetic beads. Left. Enrichment of mouse Lin- cells is performed with the LS Miltenyi columns; enrichment of human CD34+ cells is performed with the MS Miltenyi columns. The column is mounted into the MidiMACS separator on the MACS MultiStand and prepared by adding column buffer. Center. The cell suspension is added to the top of the column. Right. The cell suspension is left to flow through the column via gravity flow. Cells bound by the magnetic particles remain bound to the column in the magnetic field, whereas unbound cells are recovered into a clean tube.(C) Mouse LT-HSCs can be purified from the enriched population of Lin- cells using FACS and a 5-flurochrome strategy based on the SLAM markers. Left. Detection of Lin- cells. Center. LSK cells within the Lin- population are recognized by being c-Kit+ Sca+. Right. LT-HSCs within the LSK population are recognized by being CD150+ CD48-