Fig 1: CCL5 expression by memory cells generated on day 4.5 or day 7 pi(A) Naive CD45.1 F5 TCR-tg CD8 T cells were transferred to C57BL/6 congenic recipients (n = 5 per group) one day prior i.n. infection with VV-NP68. Mice then received one BrdU injection (2 mg i.p.) on day 4.5 (Group1) or day 7 (Group2). BrdU labeling was determined by flow cytometry on cells collected 25 days (chase) after infection.(B) Flow cytometry plots and quantification of BrdU + F5 memory cells expressing CCL5 labeled on day 4.5 and 7 with BrdU was assessed.(C and D) Flow cytometry plots and quantification of CD44+ CD49d+ BrdU + endogenous memory cells expressing CCL5 labeled on day 4.5 and 7 with BrdU was assessed in the draining lymph node (C) and in the spleen (D). *p < 0.05 (Mann-Whitney test). Data are represented as mean ± SD and are representative of 2 independent experiments.See also Figure S10 and Table S4.