Staining of 3-day unstimulated (left) and 3-day ConA activated (right) BALB/c splenocytes with 0.25 µg of Purified Rat IgG2b Isotype Control (Product # 14-4031-82) (open histogram) or 0.25 µg of Anti-Mouse CD279 (PD-1) Purified (filled histogram) followed by Anti-Rat IgG Biotin (Product # 13-4813-85)and Streptavidin PE (Product # 12-4317-87).Total viable cells were used for analysis.
Supplier Page from Thermo Fisher Scientific for CD279 (PD-1) Antibody