Fig 1: Unlike Tconv cells, iNKT cells are resistant to glucocorticoid-induced apoptosis(A) WT B6 mice were left undisturbed or restrained for 12 h, after which HMNCs and splenocytes were harvested and stained with a monoclonal antibody (mAb) to TCRß along with empty (control) or PBS-57-loaded CD1d tetramers. Representative dot plots and summary data depict hepatic and splenic iNKT cell frequencies in stressed and control mice.(B) The absolute numbers of iNKT and TCRß+PBS-57-loaded CD1d tetramer- Tconv cells were also calculated.(C) In addition, the percentages of iNKT and Tconv cells containing active caspases were determined by flow cytometry.(D) Hepatic iNKT and Tconv cells were purified from =5 mice that had been either subjected to 2, 6, or 12 h of restraint stress or left undisturbed. After obtaining cDNA, the indicated gene products were amplified by quantitative PCR. Gene expression fold changes in iNKT and Tconv cells isolated from stressed mice relative to corresponding cell populations from control animals were calculated using the 2-(??Ct) method and used to generate a heatmap.(E) Hepatic iNKT and Tconv cells were analyzed for their intracellular Bcl-2 content.(F) Hepatic Tconv cells were enumerated in Nr3c1fl and Nr3c1flLckcre mice that had been either restrained or left undisturbed.(G) Cohorts of WT B6 mice were given corticosterone (CS) or Veh in drinking water for 21 days before they were sacrificed for their livers and spleens, in which iNKT and Tconv cells were enumerated.Each symbol in (A)–(C) and (E)–(G) represents an individual mouse, and error bars represent SEM. *p < 0.05, **p < 0.01, ****p < 0.0001 by unpaired Student’s t tests. NS, not significant.