Fig 1: Subset defining genes of lamina propria CD8+CD69+CD103–and CD8+CD69+CD103+T cells. (A) Volcano plot of the expressed genes, with a nominal P value <0.99, comparing lamina propria CD8+CD69+CD103+ to CD8+CD69+CD103– T cells; selected genes are highlighted. On the x-axis, the log2 fold change (log2FC) is shown, and on the y-axis, the -log10 P value (-log10P) is shown. Gray indicates not significantly differentially expressed genes, yellow indicates genes with a log2FC >0.25 and -log10P > 10 × 10–2.5, green indicates genes with a log2FC >0.25 and -log10P < 10 × 10–2.5. (B) Representative flowcytometric dotplots, including Fluorescence Minus One (FMO) control, of Itgb2, GzmK, KLRG1, and EOMES (upper row) and quantification of the respective marker (lower row) comparing lamina propria CD8+CD69+CD103+ and CD103– T cells in healthy control subjects (n = 6–7; circles), CD patients from inflamed (diamonds) and noninflamed (triangles) ileum (paired, n = 4–6). Bars represent median and interquartile range. Comparison was performed with a paired 1-tailed t test. NS, not significant.