Fig 1: TnP acts systemically during the induction phase modulating DCs and induces regulatory cells.Splenocytes from EAE mice treated with vehicle or TnP (n = 5/group) were isolated at day 7, and analyzed. A) The MFI of MHC class II, CD40, CD80, CD86 in cDC (CD11c+CD11b+) and B) MFI of PDL-1 and PDL-2 in pDC (CD11c+B220low) were analyzed by flow cytometry (50,000 events). C) The percentage of CD4+ cells, D) and the MFI of CD18, CD40L, and CD69 in the CD4+ gate were analyzed by flow cytometry. E, F, G) The percentages of FOXP3-positive CD4+CD25+ Treg, IL-4-positive CD4 Th2 cells and CD5-positive CD19+CD1d+ Breg cells were analyzed by flow cytometry. Values in the bar graphs are the mean ± SEM. *p < 0.05 and **p < 0.01 compared with vehicle-treated EAE-mice.