Anti-Endothelin A Receptor/ET-A antibody ab85163 from Abcam

Product Specs
ItemAnti-Endothelin A Receptor/ET-A antibody
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab85163
HostRabbit
ApplicationsWestern blot
ClonalityPolyclonal
Conjugate/TagUnconjugated
IsotypeIgG
Antibody TypePrimary
Concentration0.5 mg/ml
Quantity100 UG
Gene NameEDNRA
ReactivityRat
TargetEndothelin A Receptor/ET-A

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+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Endothelin A (ETA) Receptors Contribute to Senescence of Brain Microvascular Endothelial CellsCanadian Journal of Physiology and PharmacologyDecember 1, 2022Yasir Abdul, Eda Karakaya, Raghavendar Chandran, Sarah Jamil, Adviye Ergulfollowed by overnight incubation at 4°C with respective ET receptor antibodies (anti-ETA receptor; ab85163, Abcam or anti-ETB receptor; AER002, Alomone Labs) or senescence marker proteins p16INK4a (PA1–30670,

(2) The EDN1/EDNRA/β‑arrestin axis promotes colorectal cancer progression by regulating STAT3 phosphorylationInternational Journal of OncologyNovember 28, 2022Yeo-Jin Lee, Eunsun Jung, Jinhyeon Choi, Jin-Seong Hwang, Eun-Jeong Jeong, Yuna Roh, Hyun Seung Ban, Sunhong Kim, Seon-Kyu Kim, Seon-Young Kim, et al.agitation. The membranes were incubated with the primary antibodies [anti-EDNRA (1:1,000; cat. no. ab85163, Abcam), anti-EDN1 (1:1,000; cat. no. ab2786, Abcam), anti-phosphorylated (p-) STAT3 (1:1,000; cat.

(3) Endothelin-axis antagonism enhances tumor perfusion in pancreatic cancerCancer LettersSeptember 1, 2022Shailendra K. Gautam, Vipin Dalal, Balasrinivasa R. Sajja, Suprit Gupta, Mansi Gulati, Nidhi V. Dwivedi, Abhijit Aithal, Jesse L. Cox, Satyanarayana Rachagani, Yutong Liu, et al.(ETAR-AER001 1:400; ETBR-AER002 1:400) and for immunofluorescence assay; we used antibodies from Abcam (ETAR-ab85163 1:400; ETBR-117529 1:400). Antibody against endothelin-1 (ET-1 1:1000 for WB and 1:400 for IF) was used

(4) Porphyromonas gingivalis infection upregulates the endothelin (ET) system in brain microvascular endothelial cellsCanadian Journal of Physiology and PharmacologyJuly 1, 2022Eda Karakaya, Yasir Abdul, Nityananda Chowdhury, Bridgette Wellslager, Sarah Jamil, Onder Albayram, Özlem Yilmaz, Adviye Ergulbovine serum albumin followed by incubation for 12 hours at 4°C with ETA receptor primary antibody (ab85163, Abcam), ETB receptor primary antibody (AER002, Alomone labs), p16INK4a polyclonal antibody (PA1–30670),

(5) Rho kinase inhibition ameliorates vascular remodeling and blood pressure elevations in a rat model of apatinib-induced hypertensionJournal of hypertensionApril 1, 2022Li C, Ma L, Wang Q, Shao X, Guo L, Chen J, Wang W, Yu J.(1 : 20000, ab125025, Abcam), MLCP (1 : 1000, ab32519, Abcam), endothelin A receptor (ETA, 1 : 900, ab85163, Abcam), and collagen I (Col I, 1 : 500, ab6308, Abcam). The membranes were incubated with horseradish

(6) The Involvement of Endothelin Pathway in Chronic Psychological Stress-Induced Bladder Hyperalgesia Through Capsaicin-Sensitive C-Fiber AfferentsJournal of Inflammation ResearchFebruary 22, 2022Chuying Qin, Yinhuai Wang, Sai Li, Yuanyuan Tang, Yunliang Gaogroup and then transferred to a polyvinylidene fluoride membrane. After the primary antibodies ETAR (ab85163, Abcam, MA, USA) and GAPDH (60004-1-Ig, Proteintech, Wuhan, China) and appropriate Secondary antibody

(7) NOX4/Src regulates ANP secretion through activating ERK1/2 and Akt/GATA4 signaling in beating rat hypoxic atriaThe Korean Journal of Physiology & PharmacologyMarch 1, 2021Cheng-zhe Wu, Xiang Li, Lan Hong, Zhuo-na Han, Ying Liu, Cheng-xi Wei, Xun Cui(Monmouth Junction, NJ, USA). Anti-NOX4 and anti-GATA4 antibodies were obtained from Abcam (ab133303, ab85163, ab117529, and ab84593, respectively; Cambridge, UK). The anti-Src antibody was from Millipore (04-889;

(8) Quercetin attenuates reduced uterine perfusion pressure -induced hypertension in pregnant rats through regulation of endothelin-1 and endothelin-1 type A receptorLipids in Health and DiseaseDecember 1, 2020Xia Sun, Shuping Zhang, Haitao Songincubated using primary antibodies for ET-1 (1:1000, ab2786, Abcam, Shanghai, China) and ETAR (1:500, ab85163, Abcam, Shanghai, China) overnight at 4 °C. Horseradish peroxidase-conjugated secondary antibodies were

(9) Endothelin-1 (ET-1) promotes a proinflammatory microglia phenotype in diabetic conditionsCanadian journal of physiology and pharmacologySeptember 1, 2020Abdul Y, Jamil S, He L, Li W, Ergul A.bovine serum albumin followed by incubation for 12 hours at 4ºC with primary antibody anti-ETA receptor (ab85163, Abcam) or anti-ETB receptor (AER002, Alomone labs) at 1:1000 dilution or anti β-actin at 1:000 dilution.

(10) Sleeve gastrectomy ameliorates endothelial function and prevents lung cancer by normalizing endothelin-1 axis in obese and diabetic ratsWorld journal of gastroenterologyMay 28, 2020Ruze R, Xiong YC, Li JW, Zhong MW, Xu Q, Yan ZB, Zhu JK, Cheng YG, Hu SY, Zhang GY.overnight with the following primary antibodies: ET-1 (ab117757; Abcam, Cambridge, United Kingdom), ET-A (ab85163; Abcam), ET-B (ab262694; Abcam), ECE-1 (sc-376017; Santa Cruz Biotechnology, Dallas, TX, United States),

(11) Loss of Histone H3 K79 Methyltransferase Dot1l Facilitates Kidney Fibrosis by Upregulating Endothelin 1 through Histone Deacetylase 2Journal of the American Society of NephrologyFebruary 1, 2020Long Zhang, Lihe Chen, Chao Gao, Enuo Chen, Andrea R. Lightle, Llewellyn Foulke, Bihong Zhao, Paul J. Higgins, Wenzheng Zhanghistone H3 (ab1791; Abcam), HDAC2 (ab16032; Abcam), ET1 (18201; IBL International), endothelin A (ETA; ab85163; Abcam), ETB (ab117529; Abcam), RFP (632496; Clontech), GFP (632460; Clontech), FSP1 (A5114; Dako),

(12) Lipopolysaccharide-Induced Necroptosis of Brain Microvascular Endothelial Cells Can Be Prevented by Inhibition of Endothelin ReceptorsPhysiological ResearchJune 28, 2018Y. ABDUL, R. WARD, G. DONG, A. ERGULUSA), anti-IREB2 (ab106296, Abcam), anti-caspase-3 (9662, Cell Signaling Tech.), anti-ETA receptor (ab85163, Abcam) or anti-ETB receptor (AER002, Alomone labs, Jerusalem, Israel) at 1:1,000 dilution or anti β-actin

(13) Alterations in Endothelin Receptors Following Hemorrhage and Resuscitation by CenthaquinPhysiological ResearchJune 28, 2018S. BRIYAL, R. GANDHAKWALA, M. KHAN, M. S. LAVHALE, A. GULATIwashed three times with 1x TBS-Tween (TBST) and incubated with rabbit polyclonal anti-ETA receptor (ab85163, Abcam, Cambridge, MA, USA, 1:1000) or anti-ETB receptor (ab117529, Abcam, Cambridge, MA, USA, 1:1000)

(14) Chronic hypoxia alters fetal cerebrovascular responses to endothelin-1American journal of physiology. Cell physiologyAugust 1, 2017Silpanisong J, Kim D, Williams JM, Adeoye OO, Thorpe RB, Pearce WJ.(m-PBS-T20). After blocking, membranes were incubated with primary antibodies (anti-ETA at 1 ␮g/ml, Abcam, ab85163; and anti-ETB at 1:2,000, Abcam, ab117529) overnight (16 –18 h) at 4°C with gentle agitation. Membranes

(15) Reduction of Uterine Perfusion Pressure Induced Redistribution of Endothelin Receptor Type-B Between the Intima and Media Contributes to the Pathogenesis of Pregnancy-Induced HypertensionCellular Physiology and BiochemistryJuly 20, 2017Yuan Sun, Xicheng Zhang, Zhaolei Chen, Miao Xu, Minghui Ouperformed as described previously [16]. The sections were incubated overnight with anti-ETAR (Abcam, ab85163) and anti-ETBR (Abcam, ab117529) for immunohistochemistry. To evaluate the expression of ETBR in endothelial

(16) Attenuation of opioid tolerance by ET B receptor agonist, IRL-1620, is independent of an accompanied decrease in nerve growth factor in miceHeliyonJune 7, 2017Shruti Gulati, Seema Briyal, Shantel Jones, Shaifali Bhalla, Anil Gulatiwashed three times with 1x TBS-Tween (TBST) and incubated with rabbit polyclonal anti-ETA receptor (ab85163, Abcam, Cambridge, MA, 1:1000) or anti-ETB receptor (ab117529, Abcam, Cambridge, MA, 1:1000) or anti-VEGF

(17) Diabetes-mediated middle cerebral artery remodeling is restored by linagliptin: Interaction with the vascular smooth muscle cell endothelin systemLife sciencesAugust 15, 2016Yasir A, Hardigan T, Ergul A.albumin followed by incubation for 12 hours at 4°C with appropriate primary antibodies. ET-A (Abcam; cat# ab85163) and ET-B (Alomone labs; cat # AER-002) at 1:1000 dilutions or b-actin at 1:3000 dilutions were used.

(18) Endothelin-1 regulates H?-ATPase-dependent transepithelial H? secretion in zebrafishEndocrinologyMay 1, 2014Ying-Jey Guh, Yung-Che Tseng, Chao-Yew Yang, Pung-Pung Hwang

(19) Vascular Endothelial Cells Mediate Mechanical Stimulation-Induced Enhancement of Endothelin Hyperalgesia via Activation of P2X2/3 Receptors on NociceptorsJournal of NeuroscienceFebruary 13, 2013Elizabeth K. Joseph, Paul G. Green, Oliver Bogen, Pedro Alvarez, Jon D. LevineETA immunoreactivity was detected with an affinity-purified rabbit anti-ETA antibody (1:500 dilution, ab85163, Abcam) followed by incubation with an HRP-conjugated donkey anti-rabbit antibody (1:5000 dilution,

(20) Renal medullary endothelin-1 is decreased in Dahl salt-sensitive ratsAmerican journal of physiology. Regulatory, integrative and comparative physiologyAugust 1, 2011Joshua S Speed, Babbette LaMarca, Hunter Berry, Kathy Cockrell, Eric M George, Joey P Granger

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Anti-Endothelin A Receptor/ET-A antibody from Abcam

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Anti-Endothelin A Receptor/ET-A antibody from Abcam

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