Fig 1: Interferon effectors localize to TDRD3-induced SG-like foci with or without G3BP1.G3BP1-KO or WT HeLa cells were transfected with GFP-tagged TDRD3 and incubated for 12 or 24 hours. Cells were then fixed and stained for: (A) IRF3, (B) IRF7, (C) TBK1, (D) TRAF3. (E) Resulting IFA image quantification by Cell Profiler.
Fig 2: IFN-I effectors are recruited to G3BP1-induced and various stress-induced SGs.(A-C) U20S cells were treated to produce oxidative shock, heat shock or osmotic shock SGs as described in the Materials and Methods, then fixed and stained for IRF3, IRF7, TBK1, TRAF3, Sting or cGAS. IFA was then performed using deconvolution microscopy. (D) G3BP1-GFP was transiently expressed in U20S cells for 18 hrs to allow formation of SGs, which were then subjected to IFA microscopy for innate factors indicated as described in the Materials and Methods.
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