Fig 1: Active NK cells with a unique transcriptome profile. a Top two enriched gene sets (ranked by normalized enrichment score) of five different datasets from GSEA of the “Inflamed NK” cluster compared to the rest of the cells were plotted. b The expression of CD69 in the BM sample was shown as a violin plot. The y-axis represents log-normalized expression value. c Module score was calculated using up-regulated DEGs of “Active NK” (left) or “Inflamed NK” (right) cluster from BM sample and plotted via violin plots. d Up-regulated IEGs from “Active NK” cluster were plotted using heatmap of the BM sample. e The expression of CXCR4 in the BM sample was shown as a violin plot. The y-axis represents log-normalized expression value. f Percentage of CXCR4+ NK cells (gated on Lin-CD56+ cells) was evaluated via flow cytometry. g The expression of CXCR4 in CD57+/-, CD62L+/-, or NKG2A+/- CD56dim NK populations from BM was assessed via flow cytometry (top). Percentage of CXCR4+ cells within each population were quantified (bottom). n = 6 from two to five independent experiments. Paired Student’s t test was used for the statistical analysis. *P < 0.05; **p < 0.01; ***p < 0.001; n.s. stands for “not significant.” Source data for f and g are provided as a Source Data file. See also Supplementary Fig. 5
Fig 2: sNKG2DLs impair CD8+ T cell function and memory formation. (A) Human T cells derived from healthy donors were activated and treated with different concentrations of the tumor cell culture supernatant for 12 h. NKG2D expression was detected using FACS. (B) Intracellular IFN-? production, Ki67 expression and CD107a translocation of CD8+ T cells were detected by flow cytometry. (C and D) Surface markers (CD25, CD69, CD45RO, and CCR7) of CD8+ T cells were also analyzed by FACS. Data are presented as the mean ± SEM. P-values were determined using a one-way ANOVA in (A-D). *P<0.05, **P<0.01, ***P<0.001 and ****P<0.0001. s, soluble.
Supplier Page from Thermo Fisher Scientific for CD69 Antibody PE