Fig 1: The intracellular AND logic with different signaling domains.a Diagram of intracellular AND logic. b Primary human CD8+ T cells were transduced with FOS zipCAR-containing CD3? domain and RR zipCAR-containing CD28 domain. Cytotoxicity against Her2- and Axl-expressing Nalm6 was measured 24 h after adding a-Her2-SYN9 and/or a-Axl-EE zipFvs. The heatmap indicates cytotoxicity at varying zipFv concentrations (n = 3, data are represented as mean). c Cytotoxicity of CD8+ T cells transduced with FOS zipCAR-containing CD3? domain and RR zipCAR-containing 4-1BB domain. The heatmap indicates cytotoxicity at varying zipFv concentrations (n = 3, data are represented as mean). d (Left) Isolated Treg cells were transduced with two zipCAR constructs: SYN6-CD3?-P2A-FOXP3 and SYN1-CD28-P2A-puro. After puromycin selection (2 µg/mL), Treg cells were co-cultured with Her2- and Axl-expressing Nalm6 target cells (Right) The heatmap shows surface CTLA-4 expression detected after 48 h by flow cytometry at varying zipFv concentrations (a-Axl-SYN5 and a-Her2-SYN2) (n = 3, data are represented as mean).