Fig 2: Adipose tissue SRA1 gene expression is correlated with TLRs and signaling molecules. Subcutaneous adipose tissues were obtained from lean, overweight, obese individuals. In this case, mRNA expression of SRA1, TLR2, TLR3, TLR4, TLR7, IRAK1 and NF-kB was detected by real-time RT-PCR and represented as fold change over controls. (A–F) In the studied population, SRA1 transcript levels, in adipose tissue, are positively correlated with TLRs and their signaling molecules.

Fig 3: Adipose IRAK1 protein expression in adipose tissue and its correlation with SRA1 protein expression. IRAK1 protein expression was determined in adipose tissue samples from lean (normal weight; NW) and obese individuals (n = 8 for each group) using by immunohistochemistry (IHC) as described in Materials and Methods. IHC staining intensity was expressed as arbitrary units (AU) and the data (mean ± SEM) were compared between NW and obese populations using unpaired t-test and p < 0.05 was considered significant. (A) The representative IHC images are shown for NW and obese individuals. (B) IHC staining intensity data (AU). (C) Correlation between IRAK1 protein expression with SRA1.

Fig 4: Adipose SRA1 protein expression in adipose tissue. SRA1 protein expression was determined in adipose tissue samples from non-diabetic individuals with NW and obesity, as well as from persons with type-2 diabetic (T2D), as NW and those with obesity, 10 each, using by immunohistochemistry (IHC) as described in Materials and Methods. IHC staining intensity was expressed as arbitrary units (AU) and the data (mean ± SEM) were compared between NW and obese populations, with or without T2D, using unpaired t-test and p < 0.05 was considered significant. (A) The representative IHC images are shown for non-diabetic NW/obese and T2D NW/obese individuals, one each. (B) IHC staining data (AU) show the elevated adipose SRA1 expression in persons with obesity compared to NW persons, whether with or without T2D (p < 0.0001).

Fig 5: SRA1 gene expression is correlated with TLR3, TLR4, TLR7, TLR9, Myd88, TRAF6, NF-kB or IRF5 in the adipose tissues obtained from individuals with T2D (A–G).