Fig 1: PAB inhibited cell viability and increased intracellular ferrous iron and ROS.A Growth inhibitory effect of PAB in MDA-MB-231 cells and MDA-MB-468 cells. B Flow cytometric analysis of cell cycle distribution by PAB in MDA-MB-231 cells. C PAB increased intracellular ferrous iron in MDA-MB-231 cells. D PAB increased expression of TfR and FTH in MDA-MB-231 cells. E PAB increased Cy5.5-ferritin uptake in MDA-MB-231 cells, scale bars represent 100 μm. F PAB increased intracellular ROS production in MDA-MB-231 cells; scale bars represent 200 μm. G Quantitative results of ROS via FCM in MDA-MB-231 cells. H PAB increased intracellular lipid ROS production in MDA-MB-231 cells; scale bars represent 50 μm. I Lipid peroxidation via FCM in MDA-MB-231 cells. J Lapatinib and PAB inhibited cell viability in MDA-MB-231 cells. K The CI (combined index) value was calculated between lapatinib and PAB by the Chou-Talalay. L Proliferative ability of lapatinib and/or PAB treated MDA-MB-231 cells displayed by EdU assay; scale bars represent 100 μm. **P < 0.01.