Fig 2: Maturation of BDCA-1+ myDCs after exposure to melanoma cells treated with T-VEC or conditioned medium. Graphs show the expression of CD86, CD80, CD83, CD40 and MHC-I molecules (HLA-ABC) on BDCA-1+ myDCs after treatment with conditioned medium (=SN of cells treated with T-VEC for 24 or 48 h. As a negative control, BDCA-1+ myDCs were exposed to SN of untreated cells. Graphs display the percentages (red) and (normalized) MFI (blue) as median of different patient samples of at least three independent experiments. Data were analyzed using a Kruskal–Wallis test followed by Dunn’s multiple comparisons test. * p < 0.05 Abbreviations: myDCs: myeloid dendritic cells; MFI: mean fluorescence intensity; SN: supernatant; T-VEC: Talimogene laherparepvec; UT: untreated.