Fig 1: Cellular and molecular effects in AML cells treated with S63845, HDM201, and trametinib. Increase in the percentage of annexin positive apoptotic cells based on cytometric analysis in the AML cell lines MOLM-13 (A) and OCI-AML3 (B) treated for 20 h with single compounds or with combination treatment. Reduced levels of phospho-Ser-RPS6 (C) in OCI-AML3 cells treated with the indicated amounts of S63845 (S, nM) and trametinib (T, nM). Representative Western blot of total protein extracts detecting pSer-RPS6 and ß-actin. Significance is denoted for p < 0.05 (*); p < 0.005 (**).