Fig 1: Induction of IL-10 producing Bregs. (A) Spleen was harvested and processed for negative selection of CD19+ B cells. After estimation of B cells purity (>95 %), cells were stimulated with LPS (10 ug/ml) for different time periods (5h and 24h). (B) Zebra plot depicting the percentages of CD19+IL-10+ in media and LPS after 5h and 24h of stimulation. (C) Line plot showing CD19+IL-10+ B cells after 5h and 24h of LPS stimulation. (D) Zebra plot depicting the percentages of CD19+CD5+CD1dhi B10 cells and histograms depicting IL-10 production by these cells. (E) ELISA results of culture supernatants showing levels of IL-10 cytokine. The above images are indicative of one experiment and similar results were obtained in three independent experiments. The results were evaluated by ANOVA with subsequent comparisons by Student t-test for paired or nonpaired data. Statistical significance was considered as p=0.05 (*p = 0.05, **p = 0.01, ***p = 0.001) with respect to indicated groups (Mouse Image courtesy: Ms. Leena Sapra).