Fig 2: Expression of PHLDA1 and LDH release in ischemia cardiac muscle cells. a PHLDA1 mRNA expression. b PHLDA1 protein expression. c LDH release

Fig 3: Overexpression of PHLDA1 induces apoptosis. a Annexin V/PI staining of cardiac cells without transfection, with GFP transfection, or with PHLDA1 transfection. b Respective summarized data are shown in the histogram. c LDH leakage activity of cardiac cells without transfection, with GFP transfection, or with PHLDA1 transfection

Fig 4: PHLDA1 protein expression level in ICM rat model. Left ventricular tissues from control rats (left) or ICM rats after 14 days (middle) and 28 days (right) of coronary artery ligation were tested by immunohistochemical staining (a) and western blot (b). The result indicated increasing protein level of PHLDA1. Each group contains three rats