Fig 1: (Related to Fig 4).Primed elements are diverse and can acquire enhancer activity when studied in isolation. (A, B, C, D): Screenshots of the indicated gene loci with priming elements highlighted by a grey box. ATAC-Seq profile for each of the five cell stages show along with the RNA-seq forward (Fwd) and reverse (Rev) tag counts. Middle panel: expression of the gene as measured by RNA-seq of nuclear RNA with the dotted red line showing the threshold values determined as being expressed. Right panels: activity of ATAC-Seq fragments scoring positive for enhancer activity at the different developmental stages. n = 3 biologically independent replicates for Hand1, Meis1, and Spi1, n = 5 biologically independent replicates for Kdr. The presence or absence of an ATAC site is indicated as a binary code (1 = scoring positive, 0 = scoring negative). Error bars show the SD. Significant P-values are shown, P-values were calculated using Student's two-sided t test.