Fig 2: IFN-? and IFN-a trigger BAFF production from decidual stromal cells.(a) BAFF production and relative BAFF mRNA levels to medium control by isolated decidual stromal cells in response to IFN-?, IFN-a or LPS. (b) BAFF production and relative BAFF mRNA levels to medium control by cord blood mononuclear cells in response to IFN-?, IFN-a or LPS. (c) BAFF-R expression on CD105-positive stromal cells isolated from decidua basalis or decidua parietalis after culture in the presence or absence of IFN-?. (d) Histograms depict podoplanin expression on stromal cells isolated from decidua basalis or decidua parietalis and the scatter plot shows the percentage of podoplanin-positive cells after stimulation with IFN-? or not. (e) Immunofluorescence staining of podoplanin on stromal cells isolated from decidua basalis stimulated with IFN-? or not (podoplanin in green and nuclei staining with Hoechst in blue). Images from one donor in the upper panel are presented with x 10 magnification and images from a second donor in the lower panel are presented with x 25 magnification (scale bar 40 µm). Horizontal bars indicate median. *P = 0.05, **P = 0.01 and ****P = 0.0001, Kruskal-Wallis test followed by Dunn’s multiple comparison test (Fig. 3a) and Wilcoxon signed-rank test (Fig. 3d).