Fig 1: Functional characterization of distinct T cell subsets from healthy donors (Median and IQR for n = 5 of biological replicates) using three different WBA stimulation protocols.Frequency of IFN-?, TNF and IL-2 single (1+), double (2+) or triple (3+) positive CD4+ (A) and CD8+ (E) T cells after stimulation with SEB according to three different protocols (SET1-3) as described in Table 1. Difference in % cytokine secreting in parent population in healthy samples stimulated with SEB across the three WBA stimulation protocols were analysed with one-way ANOVA followed by Tukey’s comparisons test, only significant 2-tailed p values (p<0.05) are shown on the bar graphs. Pie charts show the proportion of cytokine-producing CD4+ (B-D) and CD8+ (F-H) T cells according to their polyfunctionality. Percentage under each pie chart indicates the proportion of total cytokine secreting cells.