Staining of C57BL/6 splenocytes, either unpulsed (left) or pulsed with the SIINFEKL peptide (right), with 0.125 µg of Mouse IgG1 kappa Isotype Control Purified (Product # 14-4714-82) (open histogram) or 0.125 µg of Anti-Mouse OVA (257-264) (SIINFEKL) peptide bound to H-2Kb Purified (filled histogram) followed by F (ab')2 Anti-Mouse IgG PE (Product # 12-4012). Cells in the lymphocyte gate were used for analysis.
Supplier Page from Thermo Fisher Scientific for OVA257-264 (SIINFEKL) peptide bound to H-2Kb Antibody