Fig 2: The effect of CCR2 and CX3CR1 deficiency on monocyte and CD8+ T cell activation status in the blood of L31 mice. (A) Representative flow cytometry dot plot and quantification analysis showed a decrease in the number of CD115+CD11b+ monocytes in the blood of L31/CX3CR1KO mice and an almost abolishment of CD115+CD11b+ monocytes in L31/CCR2KO mice. (B) Compared to L31 mice, the frequency and number of CD8+ T cells were reduced in the blood of L31/CX3CR1KO mice, but not affected in L31/CCR2KO mice (C) The majority of the reduced CD8+ T cells in L31/CX3CR1KO mice were of the activated subset (CD44+CD43+). (D) The frequency and number of CD8+ T cells with effector memory phenotype (CD44+CD62L-) were significantly reduced in the blood of L31/CX3CR1KO mice compared to L31 and L31/CCR2KO mice. Disease was induced by PSNL and experiments done 30 days post PSNL. All quantitative analyses are shown as number of cells per µl blood. n = 5-8/group; student’s t test; *p < 0.05; ***p < 0.001.

Fig 3: Increased frequencies of circulating monocyte-platelet aggregates (MPAs) during LCWE-induced KD vasculitis.(A) Serum levels of soluble P-selectin in PBS- or LCWE-injected WT and Mpl–/– mice (n = 6–7/group). (B and C) Representative flow cytometry plots (B) and frequencies (C) of CD61+CD11b+CD115+ MPAs in whole blood of WT and Mpl–/– mice 3 days after LCWE injection (n = 5–7/group). (D) Serum IL-1B concentration in PBS-and LCWE-injected WT and Mpl–/– mice at baseline and 3 days after injection (n = 6–7/group). Each symbol represents 1 mouse. Results presented as mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001 obtained by 2-way ANOVA with Bonferroni’s multiple-comparison test (A and D) or 2-tailed Mann-Whitney test (C).