Fig 1: Transcription Profiling in DDX1-Depleted CH12 CellsCH12 cells transduced with shCtrl or shDDX1 were analyzed after 72 hr in unstimulated (UNS) or CIT-stimulated conditions.(A) Western blot for DDX1 and Tubulin loading control.(B) Flow cytometric analysis for surface IgM and IgA expression.(C) Quantification of CSR for CIT cultures shown in (B) (average of 2 clones for each shRNA; n > 3, mean ± SD).(D) Quantitative PCR analysis of chRNA after 24 hr in UNS or CIT conditions. Unspliced µGLT and aGLT levels were normalized to unspliced ß-actin transcript and shCtrl UNS (n = 2, mean ± SD).(E) Heatmap of 120 differentially expressed genes between shCtrl or shDDX1 CH12 cells in UNS or CIT conditions. Gene expression values (FPKM) are shown (fold change =2, false discovery rate [FDR] =0.05; n = 2). Hierarchical clustering identified 11 groups of genes (clusters I–XI) with similar patterns of expression change across the different samples analyzed. See also Figure S2.