Fig 1: Non-classical CD8+ T Cells Form Memory Populations and Are Sufficient to Protect against MCMV-Induced Lethality(A and B) Frequency (A) and number (B) of non-classical CD8+ T cells in the SMG from long-term infected KbDb-/- and C57BL/6 mice (n = 12).(C) Frequency of CD8+ TRM cells (CD103+CD69+) in the SMG from long-term infected KbDb-/- and C57BL/6 mice (n = 12).(D) Representative expansion and activation of donor non-classical CD8+ T cells (CD45.2+) transferred into KbDb-/-.SJL (CD45.1+) recipients, on day 7 post-MCMV infection. Donor CD8+ TM (KLRG1-CD27+) cells were sorted from long-term infected KbDb-/- mice.(E) Frequency of donor and recipient non-classical CD8+ TEFF cells (KLRG1+CD127-) on day 7 post-infection (n = 7).(F) Fold change of donor CD8+ T cell number, assuming 100% engraftment in indicated organ on day 7 post-infection (n = 7).(G) Frequency of donor and recipient non-classical CD8+ TEFF cells (KLRG1+CD127) on day 7 post-infection (n = 8); KLRG1CD27+ donor cells were sorted from naive KbDb-/- mice prior to infection.(H) Fold change of donor CD8+ T cell number, assuming 100% engraftment in indicated organ, on day 7 post-infection (n = 8).(I) Survival of RAG1-/-KbDb-/- mice with (—) or without (- -) adoptive transfer of 5 × 104 sorted CD8+ TM cells (KLRG1CD27+) from long-term infected KbDb-/- mice (n = 6). Statistical analysis determined by Mantel-Cox test.(J) Fold-change of donor cells in the spleen (n = 12) and liver (n = 6) of RAG1-/-KbDb-/- recipients upon euthanization between 10 to 16 weeks post-infection.Data represent mean ± SEM and are pooled (A–C, E–H, and I) or representative (D and I) of at least two independent experiments. **p < 0.01, ***p < 0.001, and ****p < 0.0001.See also Figure S3.