Fig 2: Increased levels of TIM-3+PD-1+ NK cells are correlated with treatment in the SLE patients a The frequency of TIM-3+PD-1+NK cells was significantly decreased in the SLE patients that received treatment. b The MFI of TIM-3 on TIM-3+PD-1+ NK cells was significantly decreased in the SLE patients that received treatment. c There was no difference in the MFI of PD-1 on TIM-3+PD-1+NK cells before and after treatment. MFI, mean fluorescence intensity; NK, natural killer; PD-1, programmed death 1; SLE, systemic lupus erythematosus; TIM-3, T cell immunoglobulin mucin-3

Fig 3: Increased levels of TIM-3+PD-1+ NK cells are correlated with ESR and CRP in the SLE patients a The MFI of TIM-3 on TIM-3+PD-1+ NK cells was positively associated with CRP. b The MFI of PD-1 on TIM-3+PD-1+ NK cells was positively associated with ESR. c The MFI of PD-1 on TIM-3+PD-1+ NK cells was positively associated with CRP. CRP, C-reactive protein; ESR, erythrocyte sedimentation rate; MFI, mean fluorescence intensity; NK, natural killer; PD-1, programmed death 1; SLE, systemic lupus erythematosus; TIM-3, T cell immunoglobulin mucin-3

Fig 4: Associations of TIM-3 expression level of leukemic blasts with proportions of peripheral lymphocyte subtypes in AML patients. Proportions of multiple lymphocyte subsets were assessed in 27 out of 34 AML patients using flow cytometry (the percentage of B cells was only available in 20 patients). Linear regression was performed to determine associations of TIM-3 expression level of leukemic blasts with percentages of CD3+ T cells (A), CD3+CD8+ T cells (B), CD3+CD4+ T cells (C), CD4+CD25+CD127- Treg cells (E), CD19+ B cells (F) and CD16+ or CD56+ NK cells (G), as well as the ratio of CD4+/CD8+ T cells (D).

Fig 5: Increased levels of TIM-3+PD-1+NK cells are correlated with disease activity in the SLE patients a The MFI PD-1 on TIM-3+PD-1+ NK cells was positively correlated with SLEDAI score. b The MFI of PD-1 on TIM-3+PD-1+ NK cells was significantly increased in the SLE patients with anemia. c The MFI of PD-1 on TIM-3+PD-1+ NK cells was significantly increased in the SLE patients with erythrocytopenia. d The MFI of PD-1 on TIM-3+PD-1+ NK cells tended to be increased in the SLE patients with fever, but the difference was not significant. e The MFI of TIM-3 on TIM-3+PD-1+ NK cells tended to be increased in the SLE patients with arthritis, but the difference was not significant. f The MFI of TIM-3 on TIM-3+PD-1+ NK cells tended to be increased in the SLE patients with thrombocytopenia, but the difference was not significant. g The frequency of TIM-3+PD-1+NK cells in the SLE patients with CVD was significantly decreased compared with that in the SLE patients without CVD. h No correlation was found between the MFI of TIM-3 on TIM-3+PD-1+ NK cells and CVD or i between the MFI of PD-1 on TIM-3+PD-1+ NK cells and CVD. CVD, cardiovascular disease; MFI, mean fluorescence intensity; NK, natural killer; PD-1, programmed death 1; SLE, systemic lupus erythematosus; SLEDAI, SLE disease activity index; TIM-3, T cell immunoglobulin mucin-3