Fig 1: Prevalence of Trx1 and TrxR1 in HIV particles.(A) Western Blot analysis to detect Trx1 and TrxR1 in the envelope of HIV-1: HIV-1(NL4.3) particles (500 ng of p24 protein) were lysed and loaded onto a 4–12% Bis-Tris PAGE gel. Trx1 and TrxR1 were detected using the antibodies 10284-RP01 and 13093-RP01, respectively. As positive controls, commercially available Trx1 and (truncated) TrxR1 were used. M: MagicMark XP Western Protein Standard. The fig shows the results of a single representative experiment.(B) Detection of Trx1 in virus particles produced in the presence of the organotellurium compounds: HIV-1 particles which were produced in the presence of 25 μM organotellurium compounds were isolated, lysed and measured for their p24 content. Subsequently, the lysates were measured for their Trx1 content via ELISA using the antibodies MT17R6 and MT13X3-biotin. The values were normalised to the p24 values. The value for the control was then set to 100% in each individual experiment and all other values were adapted against these reference values. The data are means of three independent experiments; the error bars represent the SEM. P-values are indicated if significant (*: p < 0.05; **: p < 0.01; ***: p < 0.001).