Fig 2: Fluorescent images of the resected tumor tissues: (A) the immuno-fluorescent intensities of CD133 (green) and Nanog (red); (B) the immuno-fluorescent Sox2 (green) and Nanog (red) images; and (C) the immuno-fluorescent ABCG2 (green) and Nanog (red) images. The spheroid tumor sections from mice fed with 2 and 4 g/kg of BJ and water control were incubated with primary antibodies followed by FITC- or TRITC-conjugated secondary antibodies, before being counter-stained with DAPI (blue). Images taken from confocal microscopy were merged (scale bar, 50 µm).

Fig 3: Immunofluorescence staining showing NANOG nuclear reactivity; OCT4 reactivity is predominantly nuclear and scant in the cytoplasm; SOX2 immunoreactivity is localized to both nucleus and cytoplasm (Cy3: cyanine dye; DAPI: 4',6-diamidino-2-fenilindol).

Fig 4: Ethidium bromide-stained agarose gel electrophoresis of RT/PCR products showing clearly defined bands corresponding to NANOG, OCT4, and SOX2 amplicons of the expected size (RPS18: ribosomal protein S18).

Fig 5: The number of Sox-2, Musashi-1 and DCX immunopositive cells in the subventricular zone of control rats and animals chronically treated with chlorpromazine (CPZ). Data are presented as median with ranges. Differences between groups were statistically analyzed using non-parametric Mann–Whitney U test and they were considered significant at p < 0.05