Fig 1: Retinal transcriptomic remodeling in Gnat1rd17 mice. (a) Volcano plot showing DEGs in the neuroretina of Gnat1rd17 mice compared to WT mice. The vertical dot lines define upregulated (log2 fold change 1) and downregulated (log2 fold change −1) DEGs. The red and blue dots indicate all genes at adjusted p 0.01 and p 0.05, respectively. The grey dots represent all genes with an adjusted p 0.05. (b) The top 10 most significantly upregulated and downregulated protein-coding DEGs, as determined by adjusted p-value. (c) Representative Western blot images and relative protein expression of Gnat1 assessed by densitometry. Vinculin was used as a protein-loading control. The data represent the mean ± SEM of 5 neuroretinas of five normal WT and five Gnat1rd17 mice at the age of 10 weeks. The data shown are representative of three independent experiments. ND, not detected. Abbreviations: Cwc22—CWC22 spliceosome-associated protein; Rtkn2—rhotekin 2; Armh4—armadillo-like helical domain-containing 4; Cfap54—cilia and flagella-associated protein 54; Pccb—propionyl Coenzyme A carboxylase, beta polypeptide; Fndc1—fibronectin type III domain-containing 1; Tgm3—transglutaminase 3, E polypeptide; Pdzph1—PDZ and pleckstrin homology domains 1; Pla2g4e—phospholipase A2, group IVE; Lama3—laminin, alpha 3; Gnat1—guanine nucleotide-binding protein, alpha transducing 1; Sik2—salt inducible kinase 2; Abhd14a—abhydrolase domain-containing 14A; Gm4792—predicted gene 4792; Irf7—interferon regulatory factor 7; Haus1—HAUS augmin-like complex, subunit 1; Drd4—DA receptor D4; Mns1—meiosis-specific nuclear structural protein 1; Peli2—pellino 2; Ppargc1b—peroxisome proliferative activated receptor, gamma, coactivator 1 beta.