Fig 1: Differentiation potency of mpiPSCs, bpiPSCs and ppiPSCs in vitro. (A) a-c. EBs-like spheroids derived from mpiPSCs, bpiPSCs and ppiPSCs; d-f. EB-like spheroids spread on dishes coated with gelatin displayed distinct signs of differentiation. The immunofluorescence staining of differentiation markers ßIII-Tubulin, Sma and Keratin in EBs from piPSCs. Scale bar, 100 µm. (B) qPCR analysis of the expression of differentiation-related genes in EB-like spheroids and piPSCs cells. Data are depicted as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns, no statistical significance. (C) Generation of pPGCLCs and their morphology. Scale bar, 100 µm. (D) Immunofluorescence analysis of mesodermal markers MIXL1 and LHX1. Scale bar, 100 µm. (E) qPCR analysis of the expression of key genes for PGC specification and development in piPSCs, iMeLCs and pPGCLCs. Data are depicted as mean ± SD. (F) Analysis of the contribution of ppiPSCs to E7.5 mouse embryos. (a, b) Injection of GFP labeled ppiPSCs-9 to E7.5 mouse embryos. (c, d) Injected E7.5 mouse embryos cultured for 12 h. Scale bar, 100 µm.