Fig 1: Analysis of the RAS components in rat kidneys following HP intake. (A) qRT-PCR detection of renal transcripts of ACE, AGT, renin, and PRR. GAPDH was used as internal reference. (B) Immunoblotting analysis of renal expression of ACE, AGT, renin, fPRR and sPRR. The values indicate the corresponding densitometry analysis. ß-actin was used as an internal reference. (C) Representative images of PRR immunostaining. Arrows indicate positive staining (200×, 400×). N = 5 per group. Data are Mean ± SEM. *p < 0.05 vs. CTR.