Fig 2: The expression profile and prognostic values of SMARCC1 in HCC. a mRNA expression of SMARCC1 in normal tissues and LIHC samples from TCGA database. bThe prognostic values of SMARCC1 in HCC from Kaplan–Meier Plotter online tool. c Representative of immunohistochemical staining intensity and positive rate of SMARCC1 in HCC tissues from a tissue microarray (scan bar = 200um). d Expression of SMARCC1 protein in tumor tissues compared to adjacent tissues from a tissue microarray (scan bar = 200um). e Relationships between SMARCC1 expression and tumor grades or cytokeratin-19 (CK19) status in a local cohort of HCC patients. f Overall survival analysis in the cohort based on SMARCC1 expression levels in a local cohort of HCC patients. The values are expressed as mean ± SEM, ****P < 0.0001, ***P < 0.001, **P < 0.01 and *P < 0.05

Fig 3: Mutant and transcriptional regulation analysis of SMARCC1 in HCC. a The schematic representation of SMARCC1 mutations in HCC. b, c The mutation types of SMARCC1 (%) in HCC. d Transcription factors with high regulatory potential in HepG2 cell lines from Cistrome DB database (10 k distance to TSS). e The prognostic values of ZBTB40 in HCC from Kaplan-Meier Plotter online tool. f The relevance of SMARCC1 expression in relation to ZBTB40 expression in HCC from GEPIA database. g Relationships of SMARCC1 with DNA methylation in HCC based on TCGA database

Fig 4: The malignant behaviors of SMARCC1 in HCC. a mRNA expression of SMARCC1 in 4 HCC cell lines. b mRNA expression of SMARCC1 in HepG2 and Huh7 cells after si-RNA transfection. c The migration and invasion ability of HepG2 and Huh7 cells after si-RNA transfection. d mRNA expression of SMARCC1 in HepG2 and Huh7 cells after sh-RNA transfection. e The cell viability of HepG2 and Huh7 after sh-RNA transfection. f The migration and invasion ability of HepG2 and Huh7 cells after sh-RNA transfection. g mRNA expression of SMARCC1 of PLC-8024 and Hep3B after sh-RNA transfection. h The cell viability of PLC-8024 and Hep3B after over-expressed. i The migration and invasion ability of PLC-8024 and Hep3B after over-expressed. The values are expressed as mean ± SEM, ****P < 0.0001, ***P < 0.001, **P < 0.01 and *P < 0.05

Fig 5: SMARCC1 was associated with immune infiltration in HCC. a The correlation between SMARCC1 and tumor purity, B cell, T cells CD8 + , T cells CD4 + , macrophage, neutrophil and myeloid dendritic cell. b The infiltration profile of SMARCC1high and SMARCC1low in HCC