Fig 2: CCND1 and RAD51 are overexpressed in primary and recurrent ependymomas(A-B) CCND1 expression validated in primary (Pre-RT) and recurrent (Post-RT) supratentorium (A) and infratentorium (B) compared with normal whole brain tissue and cerebellum, respectively. qRT-PCR results are presented as mean±SD for duplicate samples. (C) CCND1 expression was more in primary and recurrent supratentorium than infratentorium. *p<0.05 by t-test. (D-E) RAD51 expression validated in primary and recurrent supratentorium (D) and infratentorium (E) compared with normal whole brain tissue and cerebellum, respectively. qRT-PCR results are presented as mean±SD for duplicate samples. (F) RAD51 expression was more in primary supratentorium than infratentorium. *p<0.05 by t-test. (G-H) IHC analyses confirmed protein levels of CCND1 (G) and RAD51 (H) in supratentorium and infratentorium. ST: supratentorium, PF: infratentorium. Scale bar: 100 µm.