Fig 2: Histological observation and light scattering property-dependent fractionation of 6 month-old sterlet testes. (A) Testes section stained with hematoxylin and eosin (HE). (B) Testes section stained with anti-vasa antibody. (C) The proportion of germ cells and somatic cells in testes. ASG, type A spermatogonia. (D, E) Bivariate histograms of flow cytometry data from dissociated testicular cells prepared from 6 month-old sterlets. Gates P1-P3 were set on the histogram and used for cell sorting. (F) Vasa-positive rates of sorted cells in gates P1-P3 and unsorted live cells [mean ± standard deviation of mean (SD)]. Means with an asterisk indicate a significant difference (n = 6, p < 0.05). (G) Microscopic observation of sorted cells using gates P1-P3 and HE stained cells from gate P1 (small rectangle). Immunofluorescent staining for detection/calculation of vasa positive cells in different gates. Blue signal, DAPI staining, Green signal, vasa antibody staining.

Fig 3: Histological observation and light scattering property-dependent fractionation of 18 month-old sterlet testes. (A) Testes sections stained with hematoxylin and eosin (HE). (B) Testes sections stained with anti-vasa antibody. (C) The proportion of various germ cell populations and somatic cells in testes. ASG, type A spermatogonia; BSG, type B spermatogonia. (D, E) Bivariate histograms of flow cytometry data from dissociated testicular cells prepared from 18 month-old sterlets. Gates P1-P3 were set on the histogram and used for cell sorting. (F) Vasa-positive rates of sorted cells in gates P1-P3 and unsorted live cells [mean ± standard deviation of mean (SD)]. Means with an asterisk indicate a significant difference (n = 6, p < 0.05). (G) Microscopic observation of sorted cells using gates P1-P3. (H) Cells from gates P1-P3 stained with anti-vasa antibody and DAPI.