Fig 2: Expression of conventional hair cell markers in the developing cochlea of the common marmoset. (A) At E96, CALB1 expression was detected only in the inner hair cells of basal turns. (B, C) At E101 and E115, CALB1 expression was detected in three rows of outer hair cells and one row of inner hair cells. (D) At P0, CALB1 expression was mainly detected in inner hair cells with slight expression in outer hair cells. (E) At E96, PVALB expression was only detected in the inner hair cells. (F, G) At E101 and E115, PVALB expression was detected in both outer hair cells and inner hair cells. (H) At P0, PVALB expression was mainly detected in inner hair cells with slight expression in outer hair cells. (I) SLC26A5 expression was not observed at E101. (J) Slight SLC26A5 expression was detected at E115. (K) At P0, strong SLC26A5 expression was observed in outer hair cells. (L) At E101, CALB2 expression was not detected in outer hair cells or inner hair cells. (M, N) At E115 and P0, CALB2 expression was detected in both outer hair cells and inner hair cells. The nuclei were counterstained with Hoechst (blue). Scale bar: 50 µm. A–C, E, F, H, I–K, N: basal turn; D and L: middle turn; G: apical turn. IHC, inner hair cells; OHC, outer hair cells. E96 (n = 5), E101 (n = 4), E115 (n = 4), and P0 (n = 3).