Fig 2: Relative abundance of Prdx6 proteins in livers of the fresh water (FW) and seawater (SW) milkfish acclimated to 28°C (white bar) and 18°C (stripe bar) for 1 week, respectively. The representative immunoblots showed single immunoreactive band of Prdx6 at about 25 kDa. The single immunoreactive band at 37 kDa was GAPDH used as the loading control. Different letters (a and b) indicate significant differences between the 28° and 18°C group, and (x and y) indicate significant differences between the FW and SW group. Values are means ± SEM, n = 6. The Student's t-test pairwise comparison was used following two-way ANOVA, P < 0.05.

Fig 3: The immunoblots and relative intensities of Prdx6 in membrane-fraction proteins of livers of the fresh water (FW) and seawater (SW) milkfish acclimated to 28°C (white bar) and 18°C (stripe bar) for 1 week, respectively. In the representative immunoblot, single immunoreactive bands at 100 and 25 kDa represent Na+, K+-ATPase (NKA), and Prdx6, respectively. The NKA was used as the loading control of the membrane fraction. Different letters (a and b) indicate significant differences between the 28° and 18°C group, and (x and y) indicate significant differences between the FW and SW group. Values are means ± SEM, n = 6. The Student's t-test pairwise comparison was used following two-way ANOVA, P < 0.05.