Fig 1: SDOS binds and regulates translation of mRNAs encoding for ciliary components. (A) RT-qPCR of Cc2D2a, Kif7 and Tmem107 mRNAs from HeLa cells upon 24 h of SDOS-eGFP or eGFP induction (ßActin used as the internal control). Data are expressed as mean ± S.E.M. from four independent experiments with technical triplicate each. Numbers above bars indicate the statistical significance (P-value), based on one-sample t-test. Red line indicates expression level of the relative eGFP control. (B) Representative results of RT-qPCR from RIP independent experiments to validate iCLIP data. RNA enrichment was normalized to a spike-in control (red line). Actin was used as negative control showing no enrichment. (C and D) WB analysis of HeLa cell extracts following SDOS-eGFP or eGFP (24 h) and sh-GFP or sh-SDOS (48 h) induction (C), with relative densitometric analysis (D), calculated by assuming protein levels of the control equal 1. The P-value in the graph indicate the statistical significance based on one-sample t-test (n = 3).