HRP Goat anti-mouse IgG (minimal x-reactivity) 405306 from BioLegend

Product Specs
ItemHRP Goat anti-mouse IgG (minimal x-reactivity)
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number405306
NCBI Gene AliasesB9-scFv, IgG, IgH, IgVH1(VSG), VH7183, VI24H
Conjugate/TagHRP
ApplicationsELISA, Immunohistochemistry (IHC), Western Blot (WB)
ReactivityMouse
Quantity500 µL
ClonePoly4053
HostGoat
Antibody TypeSecondary
NCBI Full Gene Nameimmunoglobulin heavy chain (V7183 family)
FormatAqueous buffered solution containing a carrier protein and preservatives
ClonalityPolyclonal
IsotypeIgG
TargetIgG

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Oral Tolerance to Systemic Vaccination Remains Intact without RORγt Expression in Regulatory T CellsiScienceDecember 15, 2023Nicole B. Potchen, Andrew M.F. Johnson, Kevin Hager, Jessica Graham, Phuong Van, Katelyn H. Lyn-Kew, Lakshmi Warrier, Irene Cruz Talavera, Jennifer M. Lund, James G. KublinSecondary antibodies specific for relevant Ig-subclass linked to HRP (SouthernBiotech 1033-05, BioLegend 405306, Thermo Fisher Scientific PA129288, M32407, A10551) were then incubated on plates for 1 h. TMB (Invitrogen

(2) SARS-CoV-2 spike protein S1 subunit induces potent neutralizing responses in mice and is effective against Delta and Omicron variantsFrontiers in Plant ScienceNovember 14, 2023Tarlan Mamedov, Damla Yuksel, Irem Gurbuzaslan, Burcu Gulec, Gulshan Mammadova, Aykut Ozdarendeli, Shaikh Terkis Islam Pavel, Hazel Yetiskin, Busra Kaplan, Muhammet Ali Uygut, et al.blocking solution, the wells were treated with anti-rat (Cat. no. MBS440123), anti-mouse (Cat. no. 405306, BioLegend), or anti-human IgG antibody (Cat. no. MBS440121, MyBioSource). After the plates were washed

(3) Plasma cells in human pancreatic ductal adenocarcinoma secrete antibodies against self-antigensJCI InsightNovember 8, 2023Min Yao, Jonathan Preall, Johannes T.-H. Yeh, Darryl Pappin, Paolo Cifani, Yixin Zhao, Sophia Shen, Philip Moresco, Brian He, Hardik Patel, et al.with HRP conjugated anti-human IgG (Thermo Fisher Scientific, A18811) or anti-mouse IgG (BioLegend, 405306) secondary antibody; and developed by ECL substrate. ELISA. Recombinant proteins RUVBL2 (Creative

(4) Control of CD4+ T cells to restrain inflammatory diseases via eukaryotic elongation factor 2 kinaseSignal Transduction and Targeted TherapyOctober 24, 2023Hao-Yun Peng, Liqing Wang, Jugal Kishore Das, Anil Kumar, Darby J. Ballard, Yijie Ren, Xiaofang Xiong, Paul de Figueiredo, Jin-Ming Yang, Jianxun SongCell Signaling Technology (Danvers, MA). Secondary antibodies for Western blots: HRP goat anti-mouse IgG (#405306), HRP donkey anti-rabbit IgG (#406401), HRP goat anti-rat IgG (#405405). All secondary antibodies were

(5) Partial Replacement of Diet with Dehulled Adlay Ameliorates Hepatic Steatosis, Inflammation, Oxidative Stress, and Gut Dysbiosis in Rats with Nonalcoholic Fatty Liver DiseaseNutrientsOctober 16, 2023Hsuan-Chih Huang, Pei-Ni Lee, Wen-Chih Huang, Hsin-Yi Yangat room temperature for 2 or 1 h followed by incubation with an HRP-conjugated anti-mouse antibody (405306, Biolegend, CA, USA) and anti-rabbit antibody (440640, Biolegend, CA, USA) for second antibody. Membranes

(6) The Inflammasome Pathway is Activated by Dengue Virus Non-structural Protein 1 and is Protective During Dengue Virus InfectionbioRxiv : the preprint server for biologySeptember 21, 2023Wong MP, Juan EYW, Chelluri SS, Wang P, Pahmeier F, Castillo-Rojas B, Blanc SF, Biering SB, Vance RE, Harris E.1:1000 (Adipogen, Bally-1); α-Pyrin, 1:1000 (Abcam, EPR18676); goat-α-mouse IgG HRP, 1:5000 (Biolegend, 405306); donkey-α-rabbit IgG HRP, 1:5000 (Biolegend, 405306); α-actin HRP, 1:2000 (Santa Cruz Biotechnologies,

(7) Exploring the Anti-Cancer Effects of Fish Bone Fermented Using Monascus purpureus: Induction of Apoptosis and Autophagy in Human Colorectal Cancer CellsMoleculesJuly 27, 2023Ya-Ting Chen, Shu-Jen Chen, Chun-Yi Hu, Cheng-Di Dong, Chiu-Wen Chen, Reeta Rani Singhania, Shu-Ling Hsiehdilution, cat. no. GTX100118, GeneTex, Hsinchu City, Taiwan) and Anti-α-tubulin (1:500 dilution, cat. no. 405306, BioLegend, San Diego, CA, USA) at 4 °C overnight. On the second day, the PVDF membranes were incubated

(8) Loss-of-function variants in KCTD19 cause non-obstructive azoospermia in humansiScienceJuly 21, 2023Liu J, Rahim F, Zhou J, Fan S, Jiang H, Yu C, Chen J, Xu J, Yang G, Shah W, et al.1:10000)",BioLegend,Cat# 406401; RRID: AB_2099368 "HRP Goat anti-mouse IgG (WB, 1:10000)",BioLegend,Cat# 405306; RRID: AB_315009 ,, Bacterial and virus strains,Bacterial and virus strains,Bacterial and virus strains ,, Trans5α Chemically Competent Cell

(9) Decreased efficacy of a COVID-19 vaccine due to mutations present in early SARS-CoV-2 variants of concernbioRxivJuly 4, 2023Payton A.-B. Weidenbacher, Natalia Friedland, Mrinmoy Sanyal, Mary Kate Morris, Jonathan Do, Carl Hanson, Peter S. Kimplates for 1 h at room temperature. This was then washed 3x with PBST. HRP goat anti-mouse (BioLegend 405306) was added at a 1:10,000 dilution in diluent buffer for 1 h at room temperature. This was left to incubate

(10) 4-Methylumbelliferone enhances the effects of chemotherapy on both temozolomide-sensitive and resistant glioblastoma cellsScientific ReportsJune 8, 2023Matías A. Pibuel, Daniela Poodts, Sofía A. Sias, Agustín Byrne, Silvia E. Hajos, Paula G. Franco, Silvina L. Lompardíafrom Natocor (Argentina). BrdU, monoclonal mouse anti-BrdU (317902) antibody, and goat anti-mouse HRP (405306) secondary antibody were purchased from Biolegend (San Diego, CA, USA). The goat anti-mouse cy3 (115-165-003)

(11) Sequential therapy with supercharged NK cells with either chemotherapy drug cisplatin or anti-PD-1 antibody decreases the tumor size and significantly enhances the NK function in Hu-BLT miceFrontiers in ImmunologyMay 1, 2023Kawaljit Kaur, Po-Chun Chen, Meng-Wei Ko, Ao Mei, Emanuela Senjor, Subramaniam Malarkannan, Janko Kos, Anahid Jewettanti-perforin-1 (sc-136994, Santa Cruz Biotechnology). We used anti-mouse HRP conjugated secondary antibodies (405306 BioLegend). Stain free technology (BioRad) was used for loading control. Single-cell RNA sequencing Single-cell

(12) A ferritin-based COVID-19 nanoparticle vaccine that elicits robust, durable, broad-spectrum neutralizing antisera in non-human primatesNature CommunicationsApril 17, 2023Payton A.-B. Weidenbacher, Mrinmoy Sanyal, Natalia Friedland, Shaogeng Tang, Prabhu S. Arunachalam, Mengyun Hu, Ozan S. Kumru, Mary Kate Morris, Jane Fontenot, Lisa Shirreff, et al.plates for 1 h at room temperature. This was then washed 3x with PBST. HRP goat anti-mouse (BioLegend 405306) was added at a 1:10,000 dilution in diluent buffer for 1 h at room temperature. This was left to incubate

(13) Anti-inflammatory therapy enables robot-actuated regeneration of aged muscleScience RoboticsMarch 22, 2023S. L. McNamara, B. R. Seo, B. R. Freedman, E. B. Roloson, J. T. Alvarez, C. T. O’Neill, H. H. Vandenburgh, C. J. Walsh, D. J. Mooneydetection reagent (34577, Thermo Fisher Scientific). Secondary antibodies used were goat anti-mouse IgG (405306, BioLegend) and donkey anti-rabbit IgG (406401, BioLegend). Densitometry analysis was performed using

(14) An innate pathogen sensing strategy involving ubiquitination of bacterial surface proteinsScience AdvancesMarch 22, 2023Shruti Apte, Smita Bhutda, Sourav Ghosh, Kuldeep Sharma, Thomas E. Barton, Soham Dibyachintan, Osheen Sahay, Suvapriya Roy, Akash Raj Sinha, Harikrishna Adicherla, et al.horseradish peroxidase (HRP)–tagged anti-rabbit (BioLegend, 406401), HRP-tagged anti-mouse (BioLegend 405306), anti-rabbit Alexa Fluor 488 (Invitrogen, A27206), anti-rabbit Alexa Fluor 555 (Invitrogen, A31572),

(15) Active tissue adhesive activates mechanosensors and prevents muscle atrophyNature MaterialsFebruary 1, 2023Sungmin Nam, Bo Ri Seo, Alexander J. Najibi, Stephanie L. McNamara, David J. Mooneyantibody (1:5000, 115-035-206, Jackson Immuno Research) for puromycin, goat anti-mouse for MuRF1 (1:5000, 405306, BioLegend), and donkey anti-rabbit (1:5000, 406401, BioLegend) for the rest of proteins. Densitometry

(16) Carnitine octanoyltransferase is important for the assimilation of exogenous acetyl-L-carnitine into acetyl-CoA in mammalian cellsThe Journal of biological chemistryFebruary 1, 2023Hsu J, Fatuzzo N, Weng N, Michno W, Dong W, Kienle M, Dai Y, Pasca A, Abu-Remaileh M, Rasgon N, et al.(minimal x-reactivity) (Biolegend #406401), and HRP Goat anti-mouse IgG (minimal x-reactivity) (Biolegend #405306). Fatty acid analysis Fatty acids were derivatized into methyl esters following a published procedure

(17) T7Max transcription systemJournal of Biological EngineeringJanuary 23, 2023Christopher Deich, Brock Cash, Wakana Sato, Judee Sharon, Lauren Aufdembrink, Nathaniel J. Gaut, Joseph Heili, Kaitlin Stokes, Aaron E. Engelhart, Katarzyna P. AdamalaTBST containing horseradish peroxidase-conjugate goat anti-mouse IgG1 secondary antibodies (Biolegend 405306) diluted at 1:5000 and incubated on a horizontal rocker for 60 minutes. After incubation with secondary

(18) Long-term exposure to house dust mites accelerates lung cancer development in miceJournal of Experimental & Clinical Cancer ResearchJanuary 21, 2023Dongjie Wang, Wen Li, Natalie Albasha, Lindsey Griffin, Han Chang, Lauren Amaya, Sneha Ganguly, Liping Zeng, Bora Keum, José M. González-Navajas, et al.goat anti-rabbit IgG-HRP (Cell Signaling Technology, Cat# 7074), and goat anti-mouse IgG-HRP (BioLegend, Cat# 405306) and developed in SuperSignal West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific, Cat#

(19) Simplified Purification of Glycoprotein-Modified Ferritin Nanoparticles for Vaccine DevelopmentBiochemistryJanuary 17, 2023Payton Weidenbacher, Sriharshita Musunuri, Abigail E. Powell, Shaogeng Tang, Jonathan Do, Mrinmoy Sanyal, Peter S. Kimthree times again with PBST using an ELx 405 Bio-Tex plate washer. HRP goat anti-mouse IgG1 (BioLegend 405306) was added at a 1:7500 dilution in diluent buffer for 90 min at room temperature. After incubation with

(20) SARS-CoV-2 Delta (B.1.617.2) variant replicates and induces syncytia formation in human induced pluripotent stem cell-derived macrophagesPeerJJanuary 1, 2023Thaweerattanasinp T, Wanitchang A, Saenboonrueng J, Srisutthisamphan K, Wanasen N, Sungsuwan S, Jongkaewwattana A, Chailangkarn T.(1:1000 dilution; in-house) and secondary goat anti-mouse IgG conjugated with HRP (1:4000 dilution; Cat# 405306; BioLegend, San Diego, CA, USA). Viral titers were calculated by the Spearman-Karber method and expressed

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10/21/2015Detecting Mouse Serum IgG Antibody yougang zhaiThis product was used to test if our pseudovirus can induce anti-target protein L1 antibody response after vaccination. Mice were immunized with Pseudoviruses for 2 times at 2 week intervals. Two ... Read Review

HRP Goat anti-mouse IgG (minimal x-reactivity) from BioLegend

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HRP Goat anti-mouse IgG (minimal x-reactivity) from BioLegend

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