Fig 1: Validation of immobilization associated genes: immunohistochemistry. Quantification of immunohistochemistry staining of PCK1 (panel A), AGPAT-9 (panel B), P4HB (panel C), and HSP47 (panel D) in the posterior knee capsule from the rat time course experiment. The average count of each field between two independent examiners is plotted along with a median line for each experimental group. Statistical significance (p < 0.05) in levels of protein staining is indicated with*. Values below individual graphs represent pre-processed median mRNA expression levels for the corresponding probe sets