Fig 1: CD8+CD69+CD103–T cell characterization. (A) Representative flow dotplot of Itgb2high and CD103+ expression within lamina propria (LP) CD8+CD69+ T cells. (B) Representative flow dotplot of Itgb2high and GzmK (left) and KLRG1 (right) coexpression within LP CD8+CD69+CD103– T cells. (C) Quantification of GzmK and KLRG1 within Itgb2+/highCD103–, Itgb2–/lowCD103+, and Itgb2–/lowCD103– LP CD8+CD69+ T cells for healthy control subjects (n = 6–7; circles) and CD patients from inflamed (diamonds) and noninflamed (triangles) ileum (paired, n = 4–6). Bars represent median and interquartile range. Comparison was made with a 1-way analysis of variance. (D) Representative flow dotplot including Fluorescence Minus One (FMO) control and quantification of PD-1 within Itgb2highCD103– and Itgb2–/lowCD103+ LP CD8+CD69+ T cells. Symbols and n as per panel C. Bars represent median and interquartile range. Comparison was made with a paired 2-tailed t test. (E) Quantification of Itgb2high and KLRG1 within epithelial CD8+CD69+CD103– T cells. Symbols as per panel C. Healthy control subjects: n = 7; CD patients: n = 4. Bars represent median and interquartile range.