Fig 2: DUSP6 levels are higher in EOC tissue than normal adjacent tissue, and correlate with HE4 tissue levels(A). DUSP6 mean and maximum intensity staining is higher in serous EOC tissue (n=40) than in normal adjacent tissue (NAT) (n=7). Error bars represent standard deviation. **p<0.005 (B) Representative images of NAT and serous EOC DUSP6 staining. (C) Correlation of DUSP6 and HE4 mean intensity. (D) Correlation of DUSP6 and HE4 integrated optical density (IOD).

Fig 3: HE4 upregulates expression of DUSP6 in peripheral CD8+ T cells. Two-color flow cytometric analysis of PBMCs following 24-h incubation with 0.01 mg/mL of rHE4 (HE4) or vehicle (CTR). (A) 2D-scatterplots of DUSP6 (Alexa Fluor 647) and CD4 or CD8 (FITC) are shown. Numbers on the plots indicate mean percentages of the cell populations of the each tetrameric area from four independent experiments. (B) A bar graph from flow cytometric analyses using PBMCs from four individual donors. The mean using ± SEM are shown. *p < 0.01.

Fig 4: HE4 upregulates expression of DUSP6 in PBMCs. (A) DUSP6 transcription in response to a 6-hr incubation with 0.01 mg/mL rHE4 (HE4) or equivalent amount of PBS (CTR) were evaluated by triplicated trials of real time PCR using PBMCs from four individual donors. (B) Two-color flow cytometric analysis of PBMC following 24-h incubation with 0.01 mg/mL of rHE4 (HE4) or vehicle (CTR). 2D-scatterplots of DUSP6 (Alexa Fluor 647) and CD3 or CD56 (FITC) are shown. Numbers on the plots indicate mean percentages of the cell populations of the each tetrameric area from four independent experiments. (C) Bar graphs from flow cytometric analyses using PBMCs from four individual donors. The mean ± SEM are shown. *p < 0.05, **p < 0.01.