Fig 1: Identification of the Human SSC Line(A) RT-PCR revealed gene expression of GFRA1, GPR125, THY1, UCHL1, PLZF, RET, and VASA in the human SSC line. RNA, without RT but with PCR, was used as a negative control, and ACTB served as the loading control of total RNA. (B–J) Immunocytochemistry showed the expression of GFRA1 (B), GPR125 (C), THY1 (D), VASA (E), UCHL1 (F), PLZF (G), RET (H), SV40 (I), and isotype IgGs (J) in the human SSC line. (B–J) Scale bars, 10 µm.