Purified anti-mouse/rat IL-1beta 503501 from BioLegend

Product Specs
ItemPurified anti-mouse/rat IL-1beta
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number503501
NCBI Gene AliasesIL-1beta, Il-1b
Quantity50 µg
HostArmenian Hamster
IsotypeIgG
CloneB122
Conjugate/TagUnconjugated
ApplicationsELISA Capture, ELISPOT Capture, Depletion, Immunohistochemistry (IHC)-F, Immunoprecipitation (IP), Neutralization, Western Blot (WB)
FormatPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
ReactivityMouse, Rat
Antibody TypePrimary
NCBI Full Gene Nameinterleukin 1 beta
Concentration0.50 mg/mL
ImmunogenE. coli -expressed, recombinant mouse IL-1β
ClonalityMonoclonal
TargetIL-1beta

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Leishmania mexicana centrin knockout parasites promote M1-polarizing metabolic changesiScienceSeptember 15, 2023Greta Volpedo, Thalia Pacheco-Fernandez, Timur Oljuskin, Hannah L. Markle, Nazli Azodi, Shinjiro Hamano, Greg Matlashewski, Sreenivas Gannavaram, Hira L. Nakhasi, Abhay R. SatoskarC18.2",Biolegend,"Cat.No. 511802, RRID: AB_2123769" "Purified anti-mouse / rat IL-1β, clone B122",Biolegend,"Cat.No. 503502, RRID: AB_315287" "Biotin anti-mouse IL-12/IL-23 p40, clone C17.8",Biolegend,"Cat.No. 505302, RRID:

(2) Lymph Leakage Promotes Immunosuppression by Enhancing Anti-Inflammatory Macrophage PolarizationFrontiers in ImmunologyMay 19, 2022Andrés A. Herrada, Alexandra Olate-Briones, Rodrigo Lazo-Amador, Chaohong Liu, Bairon Hernández-Rojas, Gonzalo Riadi, Noelia EscobedoIL-1β levels were measured by using antibodies specific for the mouse cytokine (capture mAb: Cat No. 503502 and detection mAb: Cat No. 515801, BioLegend), following manufacturer’s protocol. Samples were measured

(3) Enhanced pro-BDNF-p75NTR pathway activity in denervated skeletal muscleLife sciencesDecember 1, 2021Aby K, Antony R, Eichholz M, Srinivasan R, Li Y.sc-8301), IL-6 (1:500 Santa Cruz, sc-5731), TGF-β (1:1000 Biolegend, 141402), IL-1β (1:1000 Biolegend, 503502).The membranes were incubated at 4°C overnight (approximately 16 hours) on a rocker. Primary antibodies

(4) A genotype-phenotype screening system using conditionally immortalized immature dendritic cellsSTAR protocolsSeptember 17, 2021Zhao L, Liu P, Xie W, Zhang S, Thieme S, Zitvogel L, Kroemer G, Kepp O.APC-Fire750 (1/100 for flow cytometry),BioLegend,Cat# 105046 IL-1β (1/500 for ELISA),BioLegend,Cat# 503502 IL-1β biotin (1/500 for ELISA),BioLegend,Cat# 515801 IL-2 (1/500 for ELISA),BioLegend,Cat# 503702 IL-2

(5) Generation of an immortalized astrocytic cell line from Abcd1-deficient H-2KbtsA58 mice to facilitate the study of the role of astrocytes in X-linked adrenoleukodystrophyHeliyonFebruary 11, 2021Masashi Morita, Ai Toida, Yuki Horiuchi, Shiro Watanabe, Masakiyo Sasahara, Kosuke Kawaguchi, Takanori So, Tsuneo Imanakapurified anti-mouse-IL-6 antibody (BioLegend 504501) or purified anti-mouse/rat-IL-1β antibody (BioLegend 503501) as capture antibody. After washing with PBS containing 0.05% Tween-20 and blocking with 1% BSA in PBS

(6) Short-Term Combined Exercise Improves Inflammatory Profile in the Retina of Obese MiceInternational journal of molecular sciencesAugust 24, 2020Dantis Pereira de Campos T, da Cruz Rodrigues KC, Martins Pereira R, Morelli AP, da Rocha AL, Dos Santos Canciglieri R, Sanchez Ramos da Silva A, Rochete Ropelle E, Pauli JR, Moreira Simabuco F, et al.Cell Signaling Technology Akt (#4685), Cell Signaling Technology phospho-TAK1 (Ser412) (#9339), BioLegend (503502) anti-IL-1β, Cell Signaling Technology (#11948S) anti-TNF-α and anti-α-tubulin (2144) from Cell Signaling Technology

(7) Induction of autophagy through CLEC4E in combination with TLR4: an innovative strategy to restrict the survival of Mycobacterium tuberculosisAutophagyJune 1, 2020Pahari S, Negi S, Aqdas M, Arnett E, Schlesinger LS, Agrewala JN.Pharmingen, 554415), recombinant mouse TNF (BD Pharmingen, 554589), purified anti-mouse/rat IL1B (BioLegend, 503502), biotin anti-mouse TNF (BioLegend, 515801), recombinant mouse IL1B (BioLegend, 575109), purified rat

(8) The Agpat4/LPA axis in colorectal cancer cells regulates antitumor responses via p38/p65 signaling in macrophagesSignal transduction and targeted therapyMarch 27, 2020Zhang D, Shi R, Xiang W, Kang X, Tang B, Li C, Gao L, Zhang X, Zhang L, Dai R, et al.including anti-CD4 (#100401, BioLegend, China), anti-CD8a (#100775, BioLegend, China), anti-IL1-β (#503502, BioLegend, China), anti-IL-6 (#504506, BioLegend, China), and anti-TNFα (#506325, BioLegend, China)

(9) Maternal and early postnatal immune activation produce sex-specific effects on autism-like behaviors and neuroimmune function in miceScientific ReportsDecember 1, 2019William A. Carlezon et al.Enzyme-linked immunosorbent assay (ELISA) Cytokine analysis for IL-1β was performed using a sandwich ELISA kit (#503501 BioLegend, San Diego, CA) according to the manufacturer’s protocol. Serially diluted standards (recombinant

(10) Short-term strength training reduces gluconeogenesis and NAFLD in obese miceThe Journal of endocrinologyApril 1, 2019Pereira RM, Rodrigues KCDC, Anaruma CP, Sant'Ana MR, de Campos TDP, Gaspar RS, Canciglieri RDS, de Melo DG, Mekary RA, da Silva ASR, et al.anti-fatty acid synthase (sc-48357) from Santa Cruz Biotechnology and anti-TNF- α (Cat # 506101) and IL-1β (Cat # 503501) from BioLegend. The secondary antibodies used were anti-rabbit IgG, HRP-linked antibody (7074) and

(11) NLRP3 Inflammasome Mediated Interleukin-1β Production in Cancer-Associated Fibroblast Contributes to ALA-PDT for Cutaneous Squamous Cell CarcinomaCancer management and researchJanuary 1, 2019Nie S, Wang X, Wang H.

(12) Omega-3 from Flaxseed Oil Protects Obese Mice Against Diabetic Retinopathy Through GPR120 ReceptorScientific ReportsDecember 1, 2018Marcella Neves Dátilo, Marcella Ramos Sant’Ana, Guilherme Pedron Formigari, Patrícia Brito Rodrigues, Leandro Pereira de Moura, Adelino Sanchez Ramos da Silva, Eduardo Rochete Ropelle, José Rodrigo Pauli, Dennys Esper Cintraanti-Phospho-TAK1 [Ser412] (#9339) was purchased from Cell Signaling (Danvers MA USA). Antibodies anti-IL-1β (503502) and anti-TNF-α (506102) were purchased from BioLegend (San Diego, USA). Immunoprecipitation analysis For

(13) Pyroptosis Induction and DetectionCurrent protocols in immunologyAugust 1, 2018den Hartigh AB, Fink SL.microplates (e.g. Nunc Maxisorp cat. no. 442404) Anti-mouse IL-1β capture antibody (e.g. Biolegend cat. no. 503502) Carbonate coating buffer (see recipe) Wash buffer (see recipe) Blocking buffer (see recipe)

(14) Acyloxyacyl hydrolase promotes the resolution of lipopolysaccharide-induced acute lung injuryPLoS pathogensJune 1, 2017Zou B, Jiang W, Han H, Li J, Mao W, Tang Z, Yang Q, Qian G, Qian J, Zeng W, et al.

(15) Transcriptional and Molecular Pathways Activated in Mesenteric Adipose Tissue and Intestinal Mucosa of Crohn’s Disease PatientsInternational Journal of InflammationApril 9, 2017Andressa Coope et al.(ab 79056), and IL23 (ab45420) antibodies were from Abcam (Cambridge, MA). The antibody against IL1𝛽 (503502) was obtained from Biolegend (San Diego, CA). The protein molecular weight was assessed by the PageRulerTM from

(16) FSH aggravates bone loss in ovariectomised rats with experimental periapical periodontitisMolecular medicine reportsOctober 1, 2016Qian H, Guan X, Bian Z.cat. no. 2081R), monoclonal hamster anti-IL-1β (1:300; Biolegend, Inc., San Diego, CA, USA; cat. no. 503501) for 24 h at 4°C. Subsequent to incubation, the primary antibody was removed by washing the slides thrice

(17) Bone marrow-derived microglia infiltrate into the paraventricular nucleus of chronic psychological stress-loaded micePloS oneJanuary 1, 2013Ataka K, Asakawa A, Nagaishi K, Kaimoto K, Sawada A, Hayakawa Y, Tatezawa R, Inui A, Fujimiya M.phosphorylated N-methyl-D-aspartate receptor (pNMDAR, 04-1064; Millipore), 1:100 for interleukin-1β (IL-1β, 503501; BioLegend), 1:250 for Protein gene product 9.5 (PGP9.5, AB5898, Millipore), 1:250 for NeuN (Neuronal

(18) Cerebral ischaemia induced by bilateral carotid occlusion in spontaneously hypertensive rats. Supra- and infratentorial metabolism and arterial acid-base balanceJournal of the neurological sciencesAugust 1, 1977M Fujishima, Y Nakatomi, K Tamaki, J Ogata, T Omae

(19) Autacoid and anaphylactic reactivity of pulmonary and hepatic smooth musculature of the catEuropean Journal of PharmacologyJune 7, 1977Naresh Chand, Peter Eyre

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Purified anti-mouse/rat IL-1beta from BioLegend

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Purified anti-mouse/rat IL-1beta from BioLegend

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