Fig 1: Increased cytokine expression in N-WASP-null skin. a qRT-PCR analysis of indicated genes from back skin RNA of con and ko at indicated ages (n > = 3/3, two-tailed unpaired t-test). All normalized to GAPDH. b Representative toluidine blue staining for barrier defect in con and ko mice at indicated ages (P: E15.5 embryos used as positive controls; 2 days (2d) n: 6/5, 6 days(6d) n: 4/5, 3 independent experiments). c Representative immunofluorescence staining and quantification of IL-23p19 of con and ko back skin at indicated ages (n: 3/3, two-tailed paired t-test). Scale bar: 100 µm. d Representative sorting strategy of CD45+, a6H, and a6L cells from con and ko epidermal cells and qRT-PCR analysis of IL-23A and IL-17A expression in sorted cells (n: 3/3, mean ± SD, two-tailed unpaired t-test, 3 independent sortings). e Representative immunoblots and quantification of indicated proteins from epidermal lysates of 7–8-week old con and ko mice (n: 4/4, mean ± SD, two-tailed unpaired t-test). f Representative immunoblots and quantification of indicated proteins of co-precipitations of IL-23p19 with IL-12p40 from epidermal lysates of 7–8-week-old con and ko mice (n: 4/4, mean ± SD, two-tailed unpaired t-test; *p = 0.05; **p = 0.01; ***p = 0.001)