Purified anti-mouse IFN-gamma 505701 from BioLegend

Product Specs
ItemPurified anti-mouse IFN-gamma
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number505701
NCBI Gene AliasesIFN-g, If2f, Ifg
Conjugate/TagUnconjugated
ApplicationsELISA Capture, Immunohistochemistry (IHC)
CloneR4-6A2
Quantity50 µg
ReactivityMouse
IsotypeIgG1, Kappa
HostRat
FormatPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Antibody TypePrimary
NCBI Full Gene Nameinterferon gamma
Concentration0.50 mg/mL
ImmunogenPartially-purified, native mouse IFN-γ
ClonalityMonoclonal
TargetIFN-gamma

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Engineering a dual vaccine against COVID-19 and tuberculosisFrontiers in Cellular and Infection MicrobiologyOctober 26, 2023Carlyn Monèt Guthrie, Xuejuan Tan, Amber Cherry Meeker, Ashton Elisabeth Self, Lin Liu, Yong Chengperformed as we did previously (Cheng and Schorey, 2016) using anti-mouse IFNγ capture antibody (Cat. No. 505701, Biolegend) and biotin-conjugated detection antibody (Cat. No. 505803, Biolegend), followed by avidin-conjugated

(2) Loss of Anti-Tumor Efficacy by Polyamine Blocking Therapy in GCN2 Null MiceBiomedicinesOctober 5, 2023Eric T. Alexander, Erin Fahey, Otto Phanstiel, Susan K. Gilmourcoated with 0.5 µg/mL of purified anti-mouse IFN-γ capture antibody (Biolegend, San Diego, CA, USA, 505702) overnight at 4 °C. Single-cell suspensions of splenocytes or tumors were plated at 1 × 106 per well.

(3) Centrin-deficient Leishmania mexicana confers protection against New World cutaneous leishmaniasisnpj VaccinesDecember 1, 2022Greta Volpedo et al.with primary capture antibody against IL-12 (Biolegend Cat#511802, clone C18.2), IFN-γ (Biolegend Cat#505702, clone R4–6A2), IL-10 (Biolegend Cat#505002, clone JES5–16E3), and IL-4 (BD Biosciences Cat#554434,

(4) STAT1 is regulated by TRIM24 and promotes immunosuppression in head and neck squamous carcinoma cells, but enhances T cell antitumour immunity in the tumour microenvironmentBritish Journal of CancerSeptember 1, 2022Kelvin Anderson, Nathan Ryan, Divya Nedungadi, Felipe Lamenza, Michael Swingler, Arham Siddiqui, Abhay Satoskar, Puja Upadhaya, Maciej Pietrzak, Steve Oghumu(ELISA) IFN-γ ELISA was performed as described previously [27], using capture antibody (Biolegend, 505702, San Diego, CA) and detection antibody (505804). Carcinogen-induced HNSCC model Stat1+/+ and Stat1−/−

(5) Multifunctional nanoparticle potentiates the in situ vaccination effect of radiation therapy and enhances response to immune checkpoint blockadeNature CommunicationsAugust 23, 2022Ying Zhang et al.cells were single-cell cloned. Clones were selected for downstream use following IFNγ (100 U/mL; cat #505702, Biolegend) stimulation, and screened for MHC-I presentation of SIINFEKL via flow cytometry on an Attune NxT Flow Cytometer

(6) Interferon gamma constrains type 2 lymphocyte niche boundaries during mixed inflammationImmunityFebruary 8, 2022Cautivo KM, Matatia PR, Lizama CO, Mroz NM, Dahlgren MW, Yu X, Sbierski-Kind J, Taruselli MT, Brooks JF, Wade-Vallance A, et al.analyzed 2 days after Lm infection using 0.2ug/mL anti-IFNγ capture antibody (R4–6A2 Biolegend Cat. 505702) and 0.2ug/mL biotinylated anti-IFNγ detector antibody (XMG1.2 Biolegend Cat. 505804). Overall, plasma,

(7) Penfluridol targets acid sphingomyelinase to inhibit TNF signaling and is therapeutic against inflammatory autoimmune diseasesArthritis research & therapyJanuary 19, 2022Chen YH, Liu RH, Cui YZ, Hettinghouse A, Fu WY, Zhang L, Zhang C, Liu CJ.system (sc-24948A) was bought from ChemCruz. Macrophage Colony-Stimulating Factor (M-CSF, 576406), IFNγ (505702), IL-4 (504102), PE-CD25 (102008), anti-CD3 (100302), anti-CD28 (102102), anti-IL4 (504102), anti-IFNγ

(8) Mesenchymal stem cell exosome-derived miR-223 alleviates acute graft-versus-host disease via reducing the migration of donor T cellsStem cell research & therapyFebruary 26, 2021Liu W, Zhou N, Liu Y, Zhang W, Li X, Wang Y, Zheng R, Zhang Y.and anti-CD28 (5 μg/ml, 102115, Biolegend, CA, USA). The culture medium contained IFN-γ (20 ng/ml, 505701, Peprotech, NJ, USA), IL-12 (5 ng/ml, Peprotech, 505202), and anti-IL4 (5 μg/ml, Biolegend, 504101,

(9) Fate-Mapping of GM-CSF Expression Identifies a Discrete Subset of Inflammation-Driving T Helper Cells Regulated by Cytokines IL-23 and IL-1βImmunityMay 21, 2019Komuczki J, Tuzlak S, Friebel E, Hartwig T, Spath S, Rosenstiel P, Waisman A, Opitz L, Oukka M, Schreiner B, et al.

(10) T Cells Control Chemokine Secretion by KeratinocytesFrontiers in immunologyJanuary 1, 2019Rauschenberger T, Schmitt V, Azeem M, Klein-Hessling S, Murti K, Grän F, Goebeler M, Kerstan A, Klein M, Bopp T, et al.587302; 2 μg/ml) for 1 and 3 d. To some cultures, neutralizing Abs directed against IFN-γ (Biolegend 505702; 5 μg/ml) or 100 ng/ml CsA were added. All co-cultures of keratinocytes with CD8+T cells were performed

(11) Drp1 Controls Effective T Cell Immune-Surveillance by Regulating T Cell Migration, Proliferation, and cMyc-Dependent Metabolic ReprogrammingCell reportsDecember 11, 2018Simula L, Pacella I, Colamatteo A, Procaccini C, Cancila V, Bordi M, Tregnago C, Corrado M, Pigazzi M, Barnaba V, et al.RRID: AB_315316 anti-mouse IL12,Biolegend,Cat# 505303; RRID: AB_315375 anti-mouse IFNγ,Biolegend,Cat# 505702; RRID: AB_315390 anti-CD107a-PE,Biolegend,Cat# 121620; RRID: AB_2562147 anti-mouse CD4-PECy7,Biolegend,Cat#

(12) T cell-intrinsic IL-1R signaling licenses effector cytokine production by memory CD4 T cellsNature communicationsAugust 9, 2018Jain A, Song R, Wakeland EK, Pasare C.Biolegend 516401 (1:250), Anti IL-22 biotin Biolegend 516407 (1:200), Anti IFNg purified Biolegend 505702 (1:1000), Anti IFNg biotin Biolegend 505804 (1:1000), Anti IL-17F Capture antibody eBioscience 14-7473-68

(13) Autologous tumor cells/bacillus Calmette-Guérin/formalin-based novel breast cancer vaccine induces an immune antitumor responseOncotargetApril 17, 2018Godoy-Calderón MJ, Salazar V, González-Marcano E, Convit AF.αM (CD11b) (Santa Cruz Biotechnology, Cat No. sc-28664) and rat anti-mouse IFN-γ (Biolegend, Cat No.505701). The secondary antibodies used were the following: goat polyclonal anti-rabbit IgG coupled with fluorescein

(14) Egr2 and 3 Inhibit T-bet-Mediated IFN-γ Production in T CellsJournal of immunology (Baltimore, Md. : 1950)June 1, 2017Singh R, Miao T, Symonds ALJ, Omodho B, Li S, Wang P.IL-2, 100 ng/ml IL-4 (574302; BioLegend), 10 μg/ml anti–IL-12 (505303; BioLegend), 10 μg/ml anti–IFN-γ (505702; BioLegend) for Th2; and 2 ng/ml TGF-β (240-B-002; R&D Systems), 100 ng/ml IL-6 (575702; BioLegend),

(15) PTD-J domain acts as an antigen carrier for cell mediated immunityJournal of Immunotherapy ApplicationsJanuary 1, 2015Tai-Yun Lin, Yu-Hsiu Su, Chin-kai ChuangNo., MAIPS-4510) wells were coated with 0.25µg of rat anti-mouse interferonγ (clone R4-6A2, BioLegend, Cat. No. 505701) for more than 4 h at 4°C. After blocking, 1×106 RBC-depleted splenocytes (response cells) with 0.5µg

(16) In vivo antitumor activity of a recombinant IL-7/HGFbeta hybrid cytokine in miceCancer researchJanuary 1, 2011Lai L, Jin J, Goldschneider I.

(17) Lymphotoxin alpha beta 2 (membrane lymphotoxin) is critically important for resistance to Leishmania major infection in miceJournal of immunology (Baltimore, Md. : 1950)October 15, 2007Xu G, Liu D, Fan Y, Yang X, Korner H, Fu YX, Uzonna JE.

(18) Stoicheiometries of proton translocation by mitochondriaBiochemical Society transactionsJanuary 1, 1977D G Nicholls

(19) Gastrin-like activity in an Indian pythonComparative biochemistry and physiology. C: Comparative pharmacologyOctober 1, 1975Olowo-Okorun MO.

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Purified anti-mouse IFN-gamma from BioLegend

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Purified anti-mouse IFN-gamma from BioLegend

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