Fig 1: CD18 insertion at the AAVS1 locus in LAD patient-derived B lymphocytes. a Representative flow cytometry plots of EBV-immortalized LAD patient B-lymphocytes, electroporated for targeted integration of CD18 at the AAVS1 locus, showing fractions of HDR+ cells. Donor template (DT) was delivered via rAAV6. b Stacked bar plots showing quantification of HDR efficiency by flow cytometry and NHEJ efficiency by TIDE assay of SaCas9 or SaCas9-DN1S with AAVS1-CD18 rAAV6 donor template. The data are presented as the mean ± SEM of three independent electroporations. Statistics: unpaired t tests, one tailed comparing HDR (green asterisks) or NHEJ (magenta asterisks): **p < 0.01, ****p < 0.0001. c Relative frequencies of bi-allelic HDR detected by sorting the GFPdim+ and GFPbright+ cell populations as gated, based on the density and MFI of CD18 by flow cytometry. The sorted GFPbright+ population was confirmed to be bi-allelic HDR by PCR (shown in Supplementary Fig. 14). The data are presented as the mean ± SEM of three independent electroporations. Black circles indicate individual data points. Statistics: unpaired t-tests, one tailed: ****p < 0.0001. Source data is available in Source Data file