PE/Cyanine7 anti-human CD4 300512 from BioLegend

Product Specs
ItemPE/Cyanine7 anti-human CD4
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number300512
NCBI Gene AliasesCD4mut, IMD79, Leu-3, OKT4D, T4
Quantity100 tests
IsotypeIgG1, Kappa
Conjugate/TagPE_CYANINE7
HostMouse
ApplicationsFlow Cytometry (FC)
ReactivityHuman
CloneRPA-T4
Antibody TypePrimary
NCBI Full Gene NameCD4 molecule
FormatPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
ClonalityMonoclonal
TargetCD4

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Deciphering pathogenic cellular module at single-cell resolution in checkpoint inhibitor-related pneumonitisOncogeneOctober 13, 2023Pengfei Cui, Jinfeng Li, Haitao Tao, Xiaoyan Li, Liangliang Wu, Junxun Ma, Huanhuan Wang, Tingting Liu, Min Zhang, Yi Hu339203, Biosciences), Anti-Human CD3 (Pacific Blue, 300330, Biolegend), anti-human CD4 (PE/Cyanine7, 300512, Biolegend), anti-human CD8a (APC/Cyanine7, 300926, Biolegend), Anti-Human CD274 (APC, 329707, Biolegend),

(2) Skin-Grafting and Dendritic Cell “Boosted” Humanized Mouse Models Allow the Pre-Clinical Evaluation of Therapeutic Cancer VaccinesCellsAugust 18, 2023Bijun Zeng, Davide Moi, Lynn Tolley, Natalie Molotkov, Ian Hector Frazer, Christopher Perry, Riccardo Dolcetti, Roberta Mazzieri, Jazmina L. G. Cruz#,Company,Dilution LIVE/DEAD™,Aqua,NA,L34957,ThermoFisher,1:1000 a-mouse-CD45.2,FITC,QA17A26,157608,Biolegend,1:200 a-human-CD45,PerCP,2D1,368506,Biolegend,1:200 a-human-CD3,Brilliant Violet 711™,OKT3,317328,Biolegend,1:200 a-human-CD4,PE/Cyanine7,RPA-T4,300512,Biolegend,1:200 a-human-CD8a,APC/Cyanine7,SK1,344714,Biolegend,1:200 a-human-TNFa,Alexa Fluor® 647,MAb11,502916,Biolegend,1:100 a-human-IFNγ,Pacific

(3) Identification of the novel FOXP3-dependent Treg cell transcription factor MEOX1 by high-dimensional analysis of human CD4+ T cellsFrontiers in ImmunologyJuly 25, 2023Kevin Baßler, Lisa Schmidleithner, Mehrnoush Hadaddzadeh Shakiba, Tarek Elmzzahi, Maren Köhne, Stefan Floess, Rebekka Scholz, Naganari Ohkura, Timothy Sadlon, Kathrin Klee, et al.UCHT1",Biolegend,"Cat# 300452, RRID : AB_2564148" "PE/Cyanine7 anti-human CD4 Antibody, Clone RPA-T4",Biolegend,"Cat# 300512, RRID : AB_314080" "PE/Dazzle 594 anti-human CD4 Antibody, Clone RPA-T4",Biolegend,"Cat# 300548, RRID

(4) Immunoregulatory properties of erythroid nucleated cells induced from CD34+ progenitors from bone marrowPLOS ONEJune 30, 2023Julia A. Shevchenko, Roman Yu Perik-Zavodskii, Kirill V. Nazarov, Vera V. Denisova, Olga Yu. Perik-Zavodskaya, Yulia G. Philippova, Alaa Alsalloum, Sergey V. SennikovCD4-PE/Cyanine7 (mouse monoclonal antibodies against human antigen CD4, clone RPA-T4, Isotype Mouse IgG1, κ, Cat# 300512, Biolegend, San Diego, United States), CD8a-APC/Cyanine7 anti-human (mouse monoclonal antibodies against

(5) Inherited ARPC5 mutations cause an actinopathy impairing cell motility and disrupting cytokine signalingNature communicationsJune 22, 2023Nunes-Santos CJ, Kuehn H, Boast B, Hwang S, Kuhns DB, Stoddard J, Niemela JE, Fink DL, Pittaluga S, Abu-Asab M, et al.Biosciences, Cat. 555349); BV421 anti-CD4 (BD Biosciences, Cat. 565997); PE-Cy7 anti-CD4 (Biolegend, Cat. 300512); PerCP anti-CD4 (Invitrogen, Cat. MHCD0431); PerCP anti-CD4 (BD Biosciences, Cat. 566924); APC anti-CD8

(6) Structural analysis of cancer-relevant TCR-CD3 and peptide-MHC complexes by cryoEMNature CommunicationsApril 26, 2023Kei Saotome, Drew Dudgeon, Kiersten Colotti, Michael J. Moore, Jennifer Jones, Yi Zhou, Ashique Rafique, George D. Yancopoulos, Andrew J. Murphy, John C. Lin, et al.BioLegend 301048, final dilution 1:200), and human CD4 (RRID:AB_314080, PE-Cyanine7, cl. RPA-T4, BioLegend 300512, final dilution 1:200) were suspended in 50 uL Brilliant Stain Buffer (BD 566349) and added to wells

(7) Proximity-enabled covalent binding of IL-2 to IL-2Rα selectively activates regulatory T cells and suppresses autoimmunitySignal Transduction and Targeted TherapyJanuary 23, 2023Bo Zhang, Jiaqi Sun, Yeshuang Yuan, Dezhong Ji, Yeting Sun, Yudong Liu, Shengjie Li, Xingxing Zhu, Xunyao Wu, Jin Hu, et al.Brilliant Violet 510 (clone UCHT1, Biolegend, no. 300448), CD4 PE/Cyanine7 (clone RPA-T4, Biolegend, no. 300512), CD4 FITC (clone RPA-T4, Biolegend, no. 317408), CD8 Brilliant Violet 510 (clone RPA-T8, Biolegend,

(8) EC-18 prevents autoimmune arthritis by suppressing inflammatory cytokines and osteoclastogenesisArthritis research & therapyNovember 17, 2022Park JS, Yang SC, Jeong HY, Lee SY, Ryu JG, Choi JW, Kang HY, Kim SM, Hwang SH, Cho ML, et al.were used for flow cytometry for human: mouse monoclonal PE/Cyanine7 anti-human CD4 (5 μl per test, #300512) and rat monoclonal FITC IgG2a, κ isotype control Ab (#400505) were from BioLegend; mouse monoclonal

(9) High-level of intratumoral GITR+ CD4 T cells associate with poor prognosis in gastric canceriScienceNovember 7, 2022Shouyu Ke, Feng Xie, Yixian Guo, Jieqiong Chen, Zeyu Wang, Yimeng Yu, Haigang Geng, Danhua Xu, Xu Liu, Xiang Xia, et al.RRID:AB_2629574 BV510 anti-human CD8,Biolegend,Cat# 344732; RRID:AB_2564624 PE/Cyanine7 anti-human CD4,Biolegend,Cat# 300512; RRID:AB_314080 PerCP/Cyanine5.5 anti-human CD4,Biolegend,Cat# 300530; RRID:AB_893322 FITC anti-human CD4

(10) An activation to memory differentiation trajectory of tumor-infiltrating lymphocytes informs metastatic melanoma outcomesCancer CellMay 9, 2022Abhinav Jaiswal, Akanksha Verma, Ruth Dannenfelser, Marit Melssen, Itay Tirosh, Benjamin Izar, Tae-Gyun Kim, Christopher J. Nirschl, K. Sanjana P. Devi, Walter C. Olson, et al.CD69 Antibody (clone FN50),Biolegend,Cat#310906 anti-human CD4 Antibody (clone RPA-T4),Biolegend,Cat#300512 anti-human CD45 Antibody (clone HI30),Biolegend,Cat#304029 anti-human CD3 (clone SK7),Biolegend,Cat#344811 anti-human

(11) Distinct molecular and immune hallmarks of inflammatory arthritis induced by immune checkpoint inhibitors for cancer therapyNature communicationsApril 12, 2022Kim ST, Chu Y, Misoi M, Suarez-Almazor ME, Tayar JH, Lu H, Buni M, Kramer J, Rodriguez E, Hussain Z, et al.IL-17A PE-Cy7 (Biolegend, Cat No: 512315; Dilution 1:40), Anti-Human CD4 PE-Cy7 (Biolegend, Cat No: 300512; Dilution 1:50), Anti-Human CD4 APC (Biolegend, Cat No: 300514; Dilution 1:50), Anti-Human IL-10 APC

(12) Induction of robust cellular and humoral immunity against SARS-CoV-2 after a third dose of BNT162b2 vaccine in previously unresponsive older adultsNature microbiologyFebruary 1, 2022Romero-Olmedo AJ, Schulz AR, Hochstätter S, Das Gupta D, Virta I, Hirseland H, Staudenraus D, Camara B, Münch C, Hefter V, et al.

(13) DOCK8-expressing T follicular helper cells newly generated beyond self-organized criticality cause systemic lupus erythematosusiScienceJanuary 21, 2022Shiozawa S, Tsumiyama K, Miyazaki Y, Uto K, Sakurai K, Nakashima T, Matsuyama H, Doi A, Tarui M, Izumikawa M, et al.

(14) Rapid, efficient and activation-neutral gene editing of polyclonal primary human resting CD4⁺ T cells allows complex functional analysesNature methodsJanuary 1, 2022Albanese M, Ruhle A, Mittermaier J, Mejías-Pérez E, Gapp M, Linder A, Schmacke NA, Hofmann K, Hennrich AA, Levy DN, et al.(BV421, BD, cat. no. 562884; APC, BD, cat. no. 555533, clone FN50), anti-CD4 (PE-Cy7, BioLegend, cat. no. 300512´, clone RPA-T4; APC, BD, cat. no. 555349, clone RPA-T4), anti-CD38 (PE, BD, cat. no. 555460, clone HIT2),

(15) HLA-A(∗)02:01 restricted T cell receptors against the highly conserved SARS-CoV-2 polymerase cross-react with human coronavirusesCell reportsDecember 28, 2021Nesterenko PA, McLaughlin J, Tsai BL, Burton Sojo G, Cheng D, Zhao D, Mao Z, Bangayan NJ, Obusan MB, Su Y, et al.RRID:AB_10717514" CD8a-PE,Thermo Fisher,"CAT# 12-0088-42, RRID:AB_1724104" CD4-PECy7,Biolegend,"CAT# 300512, RRID:AB_314080" IFNγ-APC,Biolegend,"CAT# 506510, RRID:AB_315443" TNFα-FITC,Biolegend,"CAT# 502906,

(16) Synergistic T cell signaling by 41BB and CD28 is optimally achieved by membrane proximal positioning within parallel chimeric antigen receptorsCell Reports MedicineDecember 21, 2021Tamara Muliaditan et al.PE-Cy7 (SK1),BioLegend,"Cat# 344711, RRID:AB_2044007" Anti-human CD4 - PE/Cy7 (RPA-T4),BioLegend,"Cat# 300512, RRID:AB_314080" Anti-human CD45RA - V450 (HI100),BD Biosciences,"Cat# 560362, RRID:AB_1645575" Anti-human

(17) Expanded IL-22⁺ Group 3 Innate Lymphoid Cells and Role of Oxidized LDL-C in the Pathogenesis of Axial Spondyloarthritis with DyslipidaemiaImmune networkDecember 1, 2021Min HK, Moon J, Lee SY, Lee AR, Lee CR, Lee J, Kwok SK, Cho ML, Park SH.IL-22-positive cells in CD4+ T cells, PBMCs were immunostained using a PE-cyanine (Cy)7-conjugated anti-CD4 Ab (300512; BioLegend, San Diego, CA, USA), fixed, and permeabilised using a Cytofix/Cytoperm Plus kit (BD Biosciences).

(18) Modulating tumor infiltrating myeloid cells to enhance bispecific antibody-driven T cell infiltration and anti-tumor responseJournal of Hematology & OncologyDecember 1, 2021Jeong A. Park, Linlin Wang, Nai-Kong V. Cheunganti-human CD4-APC (BioLegend Cat# 300514, RRID:AB_314082), anti-human CD4-PE/Cyanine7 (BioLegend Cat# 300512, RRID:AB_314080), anti-human CD8-FITC (BioLegend Cat# 980908, RRID:AB_2888883), anti-human CD8-Brilliant

(19) Phase 1 trial of entinostat as monotherapy and combined with exemestane in Japanese patients with hormone receptor-positive advanced breast cancerBMC cancerNovember 24, 2021Masuda N, Tamura K, Yasojima H, Shimomura A, Sawaki M, Lee MJ, Yuno A, Trepel J, Kimura R, Nishimura Y, et al.Staining of CD3+ cells was performed using antibodies (all from BioLegend) against CD4 (Clone RPA-T4, Cat. 300512), CD8 (Clone SK-1, Cat. 344718), CD69 (Clone FN50, Cat. 310910), inducible T-cell costimulator (ICOS;

(20) Transcriptome and Function of Novel Immunosuppressive Autoreactive Invariant Natural Killer T Cells That Are Absent in Progressive Multiple SclerosisNeurology - Neuroimmunology NeuroinflammationNovember 1, 2021Belinda Carrión et al.(NOK-1 306407 Biolegend), CD3 antihuman APC- Cy7 (HIT3a 300318 Biolegend), CD4 Pe-Cy7 antihuman (RPA-T4 300512 Biolegend), CD8 antihuman eFluorNC 605 (RPA-T8 94–0088 eBioscience), CD56 antihuman PerCP-Cy5.5 (HCD56

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(1)

7/22/2016Staining PBMC With Anti-CD4 PE/Cy7 Adam GuessThis antibody was used to phenotype CD4+ T cells so that cytokine production could be analyzed. It was chosen because it is a commonly used clone, PE-Cy7 is a bright fluorochrome, and Biolegend had ... Read Review
(1)

8/26/2013PE/Cy7 Anti-Human CD4 Antibody: Strong Signal Ahmed Al-anbaryOur studies concentrate on identifying T cell memory subsets. The initial step in the identification of these cells is staining for CD4 and CD8. We have been using a very good CD4/CD8 colour ... Read Review

PE/Cyanine7 anti-human CD4 from BioLegend

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PE/Cyanine7 anti-human CD4 from BioLegend

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