Fig 2: Platelets signaling is associated with MI and poor prognosis(A) Heatmap of differentially expressed genes in matrix index high vs matrix index low samples (adjusted p value < 0.05, NMIhigh = 21, NMIlow = 72). Samples (columns) have been ordered by increasing matrix index value. Genes (rows) have been clustered based on a pearson's correlation matrix as distance and the complete clustering method.(B) Differential expression analysis was performed on the high versus low matrix index samples. Barplot illustrates top up and down-regulated genes (adj. p value < 0.05).(C) GSEA normalized enrichment scores (NES) for significantly upregulated signaling pathways between high and low matrix index in human tissue (FDR <0.05).(D) Enrichment plots from the GSEA analysis showing a strong correlation between the MI signature and platelets and TGF-beta signaling pathways.(E) Flow cytometry analysis of CD62P⁺ activated platelets in fresh blood, washing with physiologic solution and ascites fluid from patient diagnosed with stage 3 and 4 HGSOC (n = 3) (∗∗p < 0.01 one way ANOVA). Data are represented as mean ± SD.