Fig 1: Deletion of Both C16 and C4 Inhibits Recruitment of Immune Cells and Reduces Production of IL-6 and CXCL10 In VivoGroups of five BALB/c mice were infected intranasally with 1 × 105 PFU per mouse of the indicated viruses and at the indicated times p.i.(A–H) Mice were sacrificed and cells were extracted from (A and B) BAL fluid and (C–H) lungs, counted, and analyzed by flow cytometry. (A) Macrophages, (B) neutrophils, (C) NK cells, and (D) B cells were identified and quantified by fluorescence-activated cell sorting (FACS). (E–H) The number and activation status (CD69+) of CD4+ and CD8+ T cells were analyzed by FACS.(I and J) At the indicated times p.i., mice were killed and BAL fluid was extracted, and levels of (I) IL-6 and (J) CXCL10 were measured by ELISA (n = 5). Data are represented as means of cell counts ± SD. *p < 0.05 and **p < 0.01 for v?C16/?C4, compared with both v?C16 and v?C16/C4-Rev. Each experiment was performed twice and one representative experiment is shown.