PE/Cyanine5 anti-mouse CD4 100409 from BioLegend

Product Specs
ItemPE/Cyanine5 anti-mouse CD4
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number100409
NCBI Gene AliasesL3T4, Ly-4
Quantity25 µg
ReactivityMouse
CloneGK1.5
ApplicationsFlow Cytometry (FC)
IsotypeIgG2b, Kappa
HostRat
Conjugate/TagPE_CYANINE5
Antibody TypePrimary
NCBI Full Gene NameCD4 antigen
Concentration0.20 mg/mL
FormatPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
ImmunogenMouse CTL clone V4
ClonalityMonoclonal
TargetCD4

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Protocol for high-sensitivity western blot on murine hematopoietic stem cellsSTAR ProtocolsDecember 1, 2023Changzheng Li, Jingjing Guan, Yishan Li, Xiaobin Tian, Yijun Zhao, Weiming Liu, Huixuan Tian, Huiqi Tian, Yalan Yang, Meng Zhao200),BioLegend,Cat#100310; RRID: AB_312675 Anti-mouse CD4 PE-Cy5 (working dilution: 1 in 200),BioLegend,Cat#100410; RRID: AB_312695 Anti-mouse CD8a PE-Cy5 (working dilution: 1 in 200),BioLegend,Cat#100710; RRID: AB_312749 Anti-mouse/human

(2) Myelin insulation as a risk factor for axonal degeneration in autoimmune demyelinating diseaseNature NeuroscienceJuly 1, 2023Erik Schäffner, Mar Bosch-Queralt, Julia M. Edgar, Maria Lehning, Judith Strauß, Niko Fleischer, Theresa Kungl, Peter Wieghofer, Stefan A. Berghoff, Tilo Reinert, et al.were stained with CD3 and CD4. All FACS antibodies were purchased from BioLegend: CD4-PE-Cy5 (1:200, 100409, clone GK1.5) and CD3e APC (1:200, 100311, clone 145-2C11). CD4+ T cells (CD3+CD4+) were divided into

(3) Overcoming Resistance to Immune Checkpoint Inhibitor Therapy Using Calreticulin-Inducing NanoparticlePharmaceuticsJune 9, 2023Sri Vidhya Chandrasekar, Akansha Singh, Ashish RanjanBioLegend, San Diego, CA, USA: APC-Cy7 anti-CD45 (103115), PerCP anti-CD3 epsilon (100325), PE-Cy5 anti-CD4 (100410), PE-Cy7 anti-CD8a (100721), PE anti-Granzyme B (372208), BV510 anti-INFγ (505841), BV605 anti-PD-1

(4) Analyzing immune responses to varied mRNA and protein vaccine sequencesNPJ vaccinesJune 5, 2023Park HJ, Bang YJ, Kwon SP, Kwak W, Park SI, Roh G, Bae SH, Kim JY, Kwak HW, Kim Y, et al.MP6-XT22, BioLegend), IL-2-APC-labeled (Cat: 503809, clone JES6-5H4, BioLegend), CD4-PE-Cy5-labeled (Cat: 100409, clone GK1.5, BioLegend), CD8a-FITC-labeled (Cat: 100705, clone 53.6.7, BioLegend), CD44-APC-Cy7-labeled

(5) Immunization of MERS-CoV-2-infected mice with a sublethal dose of MERS-CoV or VRP-MERS-SSTAR ProtocolsJune 1, 2023Jian Zheng, Rudra Channappanavar, Stanley PerlmanCD45,BioLegend,103138 APC/Cyanine 7 anti-mouse CD3e,BioLegend,100330 PE/Cyanine 5 anti-mouse CD4,BioLegend,100410 PE/Cyanine 7 anti-mouse CD8,BioLegend,100722 APC anti-mouse IFN-γ,BioLegend,505810 FITC anti-mouse

(6) IL7 and IL7 Flt3L co-expressing CAR T cells improve therapeutic efficacy in mouse EGFRvIII heterogeneous glioblastomaFrontiers in ImmunologyFebruary 3, 2023Sheridan L. Swan, Nalini Mehta, Ekaterina Ilich, Steven H. Shen, Daniel S. Wilkinson, Alexa R. Anderson, Tatiana Segura, Luis Sanchez-Perez, John H. Sampson, Ravi V. BellamkondaBV711 (30-F11 #103147), CD3 APC/Cy7 (17A2 #100222), CD8 BV650 (53-6.7 #100742), CD4 PE/Cy5 (GK1.5 #100410), CAR tetramer Alexa 647, PD-1 BV421 (29F.1A12 #135221), TIM3 BV605 (RMT3-23 #119721), LAG3 PE (C9B7W

(7) Absence of TSC1 Accelerates CD8⁺ T cell-mediated Acute Cardiac Allograft RejectionAging and diseaseOctober 1, 2022Liang Tan, Yanan Xu, Gongbin Lan, Hongxia Wang, Zhanfeng Liang, Zhaoqi Zhang, Qianchuan Tian, Yangxiao Hou, Yong Zhao, Xubiao Xiethis study: anti-mCD4-PE (clone GK1.5, Cat#: 100408; BioLegend), anti-mCD4-PE-Cy5 (clone GK1.5, Cat#:100410; BioLegend), anti-mCD4-APC (clone GK1.5, Cat#:100412; Biolegend), anti-mCD4-APC-Cy7 (clone GK1.5, Cat#:

(8) Protocol to assess cell-intrinsic regulatory mechanisms using an ex vivo murine T cell polarization and co-culture systemSTAR protocolsSeptember 16, 2022Ma S, Hernandez JE, Huang WJM.RRID: AB_2565780 PE/Cyanine5 anti-mouse CD4 Antibody (clone GK1.5) (Dilution 1:400),BioLegend,Cat# 100410; RRID: AB_312695 "CD3ε Monoclonal Antibody (clone 145-2C11), Functional Grade (Dilution 1:1000)",Thermo Fisher Scientific

(9) Pregnancy enables antibody protection against intracellular infectionNatureJune 1, 2022Erickson JJ, Archer-Hartmann S, Yarawsky AE, Miller JLC, Seveau S, Shao TY, Severance AL, Miller-Handley H, Wu Y, Pham G, et al.antibodies (clone, cat #,) were used at 1:200 final dilution: anti-B220 (RA3-6B2, 103211), anti-CD4 (GK1.5, 100409), anti-CD8a (53-6.7, 100709), anti-CD11b (M1/70, 101209), anti-CD11c (N418, 117316), anti-CD45.2 (104,

(10) RORγt phosphorylation protects against T cell-mediated inflammationCell reportsMarch 15, 2022Ma S, Patel SA, Abe Y, Chen N, Patel PR, Cho BS, Abbasi N, Zeng S, Schnabl B, Chang JT, et al.conjugated",BioLegend,Cat# 517006; RRID: AB_10661903 "CD4 (GK1.5), PE/Cyanine5 conjugated",BioLegend,Cat# 100410; RRID: AB_312695 "CD8a (53-6.7), Alexa Fluor™ 488 conjugated",BioLegend,Cat# 100723; RRID: AB_389304 "TCR

(11) Multilayer omics analysis reveals a non-classical retinoic acid signaling axis that regulates hematopoietic stem cell identityCell Stem CellJanuary 6, 2022Katharina Schönberger et al.RRID:AB_2075049 B220-AF700,BioLegend,103232; RRID:AB_493717 Active Caspase3-PE,BD Biosciences,561011; RRID:AB_2033931 CD4-PE/Cy5,BioLegend,100410; RRID:AB_312695 CD8a-PE/Cy5,BioLegend,100710; RRID:AB_312749 H3K4me3,Millipore,07-473; RRID:AB_1977252 H3K27me3,Cell

(12) Lipid nanoparticles enhance the efficacy of mRNA and protein subunit vaccines by inducing robust T follicular helper cell and humoral responsesImmunityDecember 14, 2021Alameh MG, Tombácz I, Bettini E, Lederer K, Sittplangkoon C, Wilmore JR, Gaudette BT, Soliman OY, Pine M, Hicks P, et al.APC-Cy7",Biolegend,Cat#100222 "CD38, clone 90, AF700",Invitrogen,Cat#56-0381-82 "CD4, clone GK1.5, PE-Cy7",Biolegend,Cat#100409 "CD4, clone H29.19, PE-Cy5",Biolegend,Cat#130312 "CD4, clone RM4-5, Biotin",eBioScience,Cat#13-0042-85 "CD4,

(13) Effects of Chlorpyrifos on Serine Hydrolase Activities, Lipid Mediators, and Immune Responses in Lungs of Neonatal and Adult MiceChemical research in toxicologyJune 21, 2021Szafran BN, Borazjani A, Seay CN, Carr RL, Lehner R, Kaplan BLF, Ross MK.immune cells, lung cells were stained with extracellular antibodies for CD4 (PECy5; BioLegend Cat# 100410, RRID:AB_312695), CD8 (PECy7; BioLegend Cat# 100722, RRID:AB_312761), and CD19 (BV650; BioLegend Cat#

(14) Exposure to p40 in Early Life Prevents Intestinal Inflammation in Adulthood Through Inducing a Long-Lasting Epigenetic Imprint on TGFβCellular and molecular gastroenterology and hepatologyJanuary 1, 2021Deng Y, McDonald OG, Means AL, Peek RM Jr, Washington MK, Acra SA, Polk DB, Yan F.previously.44 Cells were labeled with R-phycoerythrin (PE)–Cyanine (Cy)5–anti-cluster of differentiation (CD)4 (100410; BioLegend, San Diego, CA) by incubation for 0.5 hours at room temperature. Then, cells were analyzed

(15) Sirt6 Regulates the Development of Medullary Thymic Epithelial Cells and Contributes to the Establishment of Central Immune ToleranceFrontiers in cell and developmental biologyJanuary 1, 2021Zhang Q, Liang Z, Zhang J, Lei T, Dong X, Su H, Chen Y, Zhang Z, Tan L, Zhao Y.clone GK1.5, 100405), CD4-PE (Biolegend, clone GK1.5, 100408), CD4-PE/Cy5 (Biolegend, clone GK1.5, 100410), CD4-APC (Biolegend, clone GK1.5, 100412), CD4-APC/Cy7 (Biolegend, clone GK1.5, 100412), CD8a-PE (Biolegend,

(16) A Single-Dose Intranasal ChAd Vaccine Protects Upper and Lower Respiratory Tracts against SARS-CoV-2CellOctober 1, 2020Hassan AO, Kafai NM, Dmitriev IP, Fox JM, Smith BK, Harvey IB, Chen RE, Winkler ES, Wessel AW, Case JB, et al.AB_2566120 PE anti-granzyme B,Invitrogen,Cat # GRB04; RRID: AB_2536538 PE/Cy5 anti-CD4,BioLegend,Cat # 100409; RRID: AB_312694 ,, Virus and bacterial Strains,Virus and bacterial Strains,Virus and bacterial Strains ,, SARS-CoV-2

(17) Ibrutinib treatment inhibits breast cancer progression and metastasis by inducing conversion of myeloid-derived suppressor cells to dendritic cellsBritish journal of cancerMarch 1, 2020Varikuti S, Singh B, Volpedo G, Ahirwar DK, Jha BK, Saljoughian N, Viana AG, Verma C, Hamza O, Halsey G, et al.

(18) Epimedium Polysaccharide Ameliorates Benzene-Induced Aplastic Anemia in MiceEvidence-based complementary and alternative medicine : eCAMJanuary 1, 2020He J, Han R, Yu G, Lavin MF, Jia Q, Cui P, Peng C.Sungene (Biotech, Tianjin, China). FITC labeled CD3+ (Catalog 100203), PE/Cy5 labeled CD4+ (Catalog 100410), PE/Cy7 labeled CD8+ (Catalog 100722), PE labeled CD80+ (Catalog 104707), and APC labeled CD86+ (Catalog

(19) Intranasal vaccination with M2e5x virus-like particles induces humoral and cellular immune responses conferring cross-protection against heterosubtypic influenza virusesPloS oneJanuary 1, 2018Lee YT, Ko EJ, Lee Y, Kim KH, Kim MC, Lee YN, Kang SM.Brefeldin A (BFA) (20μg/ml). After stimulation, lymphocytes were stained with CD4 (CD4-PE/Cy5, Cat# 100410, Biolegend) and CD8 (CD8α-PE, Cat# 100708, Biolegend) monoclonal antibodies. BD Cytofix/Cytoperm™ Plus

(20) Neutrophil swarming and extracellular trap formation play a significant role in Alum adjuvant activityNPJ vaccinesJanuary 1, 2017Stephen J, Scales HE, Benson RA, Erben D, Garside P, Brewer JM.BD Biosciences), anti-CD45.1-APC (A20; Cat. No. 558701; BD Biosciences), anti-CD4-PE-Cy5 (GK1.5; Cat. No. 100409, BioLegend), anti-Vb5.1/5.2-PE (MR9-4; Cat. No. 553190; BD Biosciences) and biotinylated anti-IgMa (DS-1;

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7/9/2019BioLegend PE/Cy5 Anti-Mouse CD4 Antibody Works Great Sue TPE/Cy5 anti-mouse CD4 Antibody from BioLegend stains reliably and produces a bright signal.

PE/Cyanine5 anti-mouse CD4 from BioLegend

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PE/Cyanine5 anti-mouse CD4 from BioLegend

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