Anti-PF4 antibody ab9561 from Abcam

Product Specs
ItemAnti-PF4 antibody
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab9561
Antibody TypePrimary
HostRabbit
Quantity100 UG
ApplicationsSandwich ELISA, Western blot
ClonalityPolyclonal
Conjugate/TagUnconjugated
Gene NamePF4
ReactivityHuman
TargetPF4

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) CXCL4-RNA Complexes Circulate in Systemic Sclerosis and Amplify Inflammatory/Pro-Fibrotic Responses by Myeloid Dendritic CellsInternational journal of molecular sciencesDecember 30, 2022Pietraforte I, Butera A, Gaddini L, Mennella A, Palazzo R, Campanile D, Stefanantoni K, Riccieri V, Lande R, Frasca L.centrifugation, and incubated overnight at 4 °C in a rotating wheel with a monoclonal anti-CXCL4 antibody ab9561 (Abcam, Cambridge, UK) (2 µg/sample) or IgG previously conjugated with 50% (w/v) Protein A agarose beads

(2) Off-target binding of an anti-amyloid beta monoclonal antibody to platelet factor 4 causes acute and chronic toxicity in cynomolgus monkeysmAbsFebruary 18, 2021Lise I. Loberg, Meha Chhaya, Alexander Ibraghimov, Edit Tarcsa, Andreas Striebinger, Andreas Popp, Lili Huang, Frank Oellien, Stefan Barghornas a natural source of PF-4. After subsequent washes, a detection antibody (pRAb-PF-4, Abcam cat.no. ab9561 100 µL/well at 1 µg/mL in PBST + 0.5% BSA) was added followed by anti-rabbit-POD conjugate (Jackson ImmunoResearch

(3) The anti-CXCL4 antibody depletes CD4(+)CD25(+)FOXP3(+) regulatory T cells in CD4+ T cells from chronic osteomyelitis patients by the STAT5 pathwayAnnals of palliative medicineSeptember 1, 2020Huang K, Ge S.CD4+ T cells from patients were co-cultured with 200 and 500 μg/L of anti-CXCL4 antibody (Abcam Cat# ab9561, RRID:AB_308720) for 24 h. To study the involvement of STAT5 pathway in the promoted effect of CXCL4

(4) Platelet proteome reveals specific proteins associated with platelet activation and the hypercoagulable state in β-thalassmia/HbE patientsScientific ReportsDecember 1, 2019Puangpaka Chanpeng, Saovaros Svasti, Kittiphong Paiboonsukwong, Duncan R. Smith, Kamonlak Leecharoenkiatof PF4 the membranes were incubated with a 1:5000 dilution of a rabbit anti-PF4 polyclonal antibody (ab9561; Abcam, Cambridge, UK) in 5% BSA for 16 hrs. The membranes were washed 3 times with 1xTBS containing

(5) CXCL4 assembles DNA into liquid crystalline complexes to amplify TLR9-mediated interferon-α production in systemic sclerosisNature communicationsMay 1, 2019Lande R, Lee EY, Palazzo R, Marinari B, Pietraforte I, Santos GS, Mattenberger Y, Spadaro F, Stefanantoni K, Iannace N, et al.by western blot After preclearing for 3 h, plasma samples were incubated with anti-CXCL4 antibody ab9561 (Abcam), or IgG previously conjugated with Dynabeads protein G (Invitrogen), for 3 h. The immunoprecipitated

(6) Neutrophil accumulation and NET release contribute to thrombosis in HITJCI InsightSeptember 20, 2018Kandace Gollomp et al.incubated with 25 μg/ml KKO, a HIT-like monoclonal antibody (19), or a polyclonal antiPF4 antibody (Abcam, Ab9561) for 1 hour at 37°C. NET digestion studies were carried out by infusing the channels with 100 U/ml DNase I

(7) Steroids Regulate CXCL4 in the Human Endometrium During Menstruation to Enable Efficient Endometrial RepairThe Journal of Clinical Endocrinology & MetabolismJune 1, 2017Jacqueline A. Maybin, Uma Thiruchelvam, Mayank Madhra, Philippa T.K. Saunders, Hilary O.D. CritchleyBurlingame, CA) and protein block (Dako, Cambridge, UK). Rabbit polyclonal CXCL4 antibody (20 µg/mL; ab9561; Abcam, Cambridge, UK) was applied overnight at 4°C. Negative controls were incubated with rabbit IgG

(8) Wnt3a is critical for endothelial progenitor cell-mediated neural stem cell proliferation and differentiationMolecular Medicine ReportsSeptember 1, 2016Yibin Du, Shuo Zhang, Tao Yu, Gongwen Du, Hui Zhang, Zongsheng Yin(Ab2721 1:1,000), rabbit monoclonal anti-p-GSK-3β (Ab5558; 1:2,000), rabbit monoclonal anti-p-β-catenin (Ab9561; 1:2,000), rabbit monoclonal anti-GSK-3β (Ab12456; 1:2,000), rabbit monoclonal anti-β-catenin (Ab4176;

(9) Chemokine degradation by the Group A streptococcal serine proteinase ScpC can be reconstituted in vitro and requires two separate domainsThe Biochemical journalSeptember 15, 2009Andrea Fritzer, Birgit Noiges, Daniela Schweiger, Angelika Rek, Andreas J Kungl, Alexander von Gabain, Eszter Nagy, Andreas L Meinke

(10) Mediators of neutrophil recruitment in human abdominal aortic aneurysmsCardiovascular researchJune 1, 2009Xavier Houard, Ziad Touat, Véronique Ollivier, Liliane Louedec, Monique Philippe, Uriel Sebbag, Olivier Meilhac, Patrick Rossignol, Jean-Baptiste Michel

(11) Platelet factor 4 is a negative autocrine in vivo regulator of megakaryopoiesis: clinical and therapeutic implicationsBloodAugust 15, 2007Lambert MP, Rauova L, Bailey M, Sola-Visner MC, Kowalska MA, Poncz M.

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Anti-PF4 antibody from Abcam

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Anti-PF4 antibody from Abcam

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